The authors reported that the same factor also partially promotes the formation of distromatic thalli of U. pertusa and other Ulva species, highlighting the potentially important role of thallusin for the normal development of green macroalgae. Pure thallusin strongly induced the differentiation of M. oxyspermum, even at very low effective concentrations between 1 fg mL-1 and 1 ag mL-1 (Matsuo et al., 2005; Gao et al., 2006). Although thallusin can be obtained from bacterial cultivations, Nishizawa et al. (2007) undertook the total syntheses of (±)-thallusin and its analogues to allow a detailed examination of thallusin’s biological activity. Whereas the compound
Purification of GFP from E.coli via Hydrophobic Interaction Chromatography Priscilla Mariel M. Cadiz Biology Department, De La Salle University, 2401 Taft Ave, Manila, Philippines *Email: firstname.lastname@example.org Protein purification is an important techniquie in order to study the function and structure of proteins. Protein purification involves the process of removing contaminants and isolating desired proteins. Chromatography is one of the methods used for purifying and isolating proteins. Green Fluorescent Protein (GFP) is the desired protein to be isolated from the bacteria Escherichia coli which allows the bacteria to fluoresce. GFP contains abundant hydrophobic sites and because of this, Hydrophobic Interaction Chromatography
Durham’s tubes filled with the broth were placed in each of the test tubes in an inverted position. Then the tubes were sterilized. After sterilization, the test tubes were inoculated with fresh bacterial cultures and one test tube was kept as control. The inoculated tubes were incubated at 37±1o C for 24-48 h (Aneja, 2001). After incubation, tubes were observed for the colour change in medium due to the production of acid and gas production.
The bacteria was plated in mid-log phase, this was done for two possible reasons. One being conjugation is highly efficient and successful during mid log phase and because kanamycin is an antibiotic that inhibits protein synthesis in growing bacteria by binding to the 30S subunit of the bacteria ribosome. This blocks the tRNA binding which stops the bacteria from making proteins for growth (Bacteriostatic). If the conjugation was successful the growing bacteria would be able to block kanamycin
All three smears were viewed under the oil immersion lens (x1000) (3). The use of oil immersion as opposed to lower magnification allowed the organisms to be viewed more clearly and determine certain characteristics of each bacteria due to the color that appeared under the microscope. The gram stain of Micrococcus leuteus (Figure 1) was gram-positive due to the purple color after being stained. The gram stain of Serratia marcescens (Figure 2) was gram-negative due to the pink color after being stained. The gram stain of M. leuteus, S. marcescens and Escherichia coli (Figure 3) was both gram-positive and gram-negative, because S. marcescens and E. coli are gram-negative (4), and M. leuteus is gram-positive.
ISOLATION, IDENTIFICATION OF STREPTOKINASE PRODUCING STREPTOCOCCUS SPECIES AND PRODUCTION OF STREPTOKINASE ABSTRACT: The objective of the study is to identify a potent streptococcal species producing streptokinase enzyme from different samples. Various food samples and soil samples were collected and analyzed for the potent streptococcal strain. They were confirmed for streptococcus species by biochemical characterization. Further, they were screened for the streptokinase activity. Among them curd sample and bore soil sample showed good activity and they were taken for further analysis and production process.
Abstract The transformation principle suggests that bacteria use DNA as their genetic material and are able to exchange their genetic material via a process of transformation. Griffith had theorised the concept of the transformation principle using two strains of bacteria and studied their ability to recombine. Avery and MacLeod followed his studies and suggested DNA was sensitive to DNase, and that the enzyme would destroy the bacteria's ability to exchange genetic material and transform into a new strain. This was then tested in the labs at Wits by second year students where they studied the transformation of ampicillin sensitive E. coli to ampicillin resistant E. coli. The results obtained there were similar to those of Avery and MacLeod,
Cyanobacteria are phototrophs that generate O2. Plant chloroplasts likely evolved from cyanobacteria by the process of endosymbiosis. Bacteria are found everywhere in the soil, water, inside your body and on your skin. A number of factors influence the rate at which bacteria grow factors such as moisture, temperature and the bacteria PH. Campbell and Reece (2010)states that, bacteria can multiple very rapidly under favourable conditions forming colonies of millions of organisms within a space as small as a drop of water.
Entering of water into the cell from the environment is influenced by solute concentration, thus the tonicity of the placement of the cell as well as the presence of partially permeable membrane (Campbell 2008). So, the solute concentration in the cell will increase the flow of water into the cell because of osmotic gradient. Therefore, in the beginning of this experiment, it was assumed that the water would be diffused into the raw potato with salt and other potatoes would be stay same as was in the beginning. 3. Experiment C: Molecular weight and rate of diffusion This experiment was aimed to understand the relation between the molecular weight of a compound and its diffusing ability.