Dilution Series Lab Report

The purpose of this experiment was to apply all knowledge gained from the entire semester while in the lab and apply it to be able to identify an unknown genus and species gram positive bacteria. Each student was given a petri dish with an unknown Gram positive bacterium inside. The petri dish with the unknown gram positive bacteria that was used in my experiment was #8. The possible bacteria inside my petri dish could be any of the following:

Higher muscle lactate accumulation and venous plasma lactate concentration is observed in a high ambient temperature compared to a moderate temperature.

To find chemical equilibrium, the following chemical equation is used in the experiment: Fe3+(aq) + SCN-(aq)  FeSCN2+(aq). When iron (III) and thiocyanate react, thiocyanoiron (III) is produced. When the concentration of all ions at equilibrium are known, the equilibrium constant can be calculated by dividing the equilibrium concentration of the reactant by the equilibrium concentration of the products. In this experiment, four equilibrium systems containing different concentrations of three different ion types (Fe(NO3)3, KSCN-, and distilled water) are made and used to determine equilibrium concentrations. The equilibrium concentrations are used to calculate the concentration that all of the components of the chemical equation are at equilibrium. Using a colorimeter or spectrometer to determine the equilibrium concentration of FeSCN2+(aq) and

The purpose of this lab was to use chemical and physical tests to identify indicators of disease in synthetic urine samples. This lab tested samples for protein levels, glucose levels, and pH levels. In a normally functioning individual, proteins cannot pass through the glomerulus; therefore proteins should not be found in urine. However, in the nephrons of individuals with Bright’s Disease, the glomerulus no longer stops all proteins from entering the urine (Giuseppe et al., 2002, pp. 357–358). Bright’s Disease is characterized by a change in the permeability of the glomerulus, which allows proteins to pass through and since the nephron has no way of reabsorbing proteins they are passed into the urine (Giuseppe et al., 2002,

NA plate: The NA plate had growth of both organism even though it was difficult to differentiate between the colonies on this

The purpose of this experiment was to insert the plasmid glow green into the bacteria with a gene of interest to produce the protein that make the bacteria glow green along with the presence of arabinose and the presence of ampicillin. Many scientists are experimenting different kind of genes that can inserted into the organism for survival. The technique of transformation was used in this experiment to give the organism a new trait that they did not possess in their life. In this experiment, the bacteria were added to four plates with certain conditions such as the existence of plasmid, ampicillin, and arabinose to see whether the bacteria grow and glow green. The results showed that the LB/amp/araC +pGLO produce a lot of colony and most

Our predictions were that for the standard protocol plates with agar and ampicillin, there would be growth and the colonies would glow under UV light. On the modified protocol on plates with agar and ampicillin where we changed the time of the heat shock, we expected less growth but the colonies would still glow. On the first negative control plate, there should have been no growth on the plate with agar and ampicillin and regular E. coli cells. On the negative control plate with just agar and E. coli cells there should have been growth but the colonies would not

The streaking technique used was a modified streaking for isolation with a heavy quadrant one. The result revealed that bacteria is alpha, with an incomplete breakdown of the medium with a susceptibility of 17mm from the bacitracin gamma hemolysis. That is why the organism represented by the bar graph was in low numbers because it was incomplete. The other test was DNase agar, it is an enzyme test used to identify if the organism has the enzyme DNA. The streaking technique is a single straight line down the middle of the plate. The unknown #257 tested positive for the enzyme DNase. Lastly, Mannitol Salt Agar (MSA) was used to test for isolation and differentiation. The streaking technique used is streaking for isolation.The unknown #257 tested positive for mannitol fermentation which means the organism is

The concentration of the stock solution 2.0x 10-4M as per label information in the lab. However, the calculated volume using the experimental data is 1.5 x 10-4M.There is 25% difference between these concentration caluclated from zero time intercept.The significant difference in the concentration drop happened by many factors.First,the rate ionization is depend the pH because pKa determines the equlibrium between p-nitrophenol and its depronated form p-nitrophenolate.Although,the pH is maintained by buffer at 7,not all of the p-nitrophenol produced by the chymotrpsin catalysis is not its depronated form.The concentration calculation is based on the absorbtion which depends on the p-nitrophenolate form.This could have contributed to the drop

Abstract — This experiment was conducted to familiarize the students with the procedures regarding distillation—to be more precise, the separation of ethanol from an alcoholic beverage—using a distillation set-up consisting of boiling chips, a Bunsen burner, a condenser, a thermometer and several other materials. In the end, it was discovered that one may actually separate a homogeneous mixture, given that the components of said mixture differ in volatility and that they utilize a complete distillation set-up and follow laboratory safety rules and regulations.

From the Unknown tube professor Cooper gave me, I scratched a little on the slant surface with the sterilized inoculating loop. Then I place it on a clean prepared slide which already had a slight drop of water. The two substances are mixed together in the middle of the slide and let dry completely. One extra step of “heat fix” is necessary to adhere everything to the surface of the slide. To start gram staining, I slightly pour crystal-violet all over the slide and let it sit for 30 seconds before wash it off with water. Next, I dye the Unknown with Gram’s iodine to create a complex only have on gram positive. The slide is rinsed by water after 30 seconds. Decolorization is the next step of the whole process. I let the alcohol flow on 45-degree angle slide within 15 seconds and wash it with water to remove colors on the surface. Lastly, the unknown is once again dyed with safranin for 1 minute then wash it off with water for the last time and dry it using bibulous paper. After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster. However, during decolorizing process, I put too much alcohol to the crystal violet-iodine complex making the color overly removed. That led to the result of my gram positive has slightly redish

Two sources of error may have affected the experiment. Firstly, the experiment required volumes of liquid to be recorded while the vapours were distilling. It was impossible to accurately measure the volume of liquid at any given moment, as the meniscus was moving side to side. Secondly, the distillation was ended while there was still liquid in to round bottom flask. The composition and volume of this liquid were unaccounted for in the calculated

Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining. Approximately 60-90% of the Gram-positive bacterial cell wall is made up of peptidoglycan and interwoven teichoic acid, while only

Inheritance matching: here, the rules of inheritance must be followed by the VNTRs. In a paternity test for example, the child must have an allele that matches one from each parent. When relationships are more distant e.g. sibling, then matches must be constant with the degree of relatedness.

Citrate test- Fresh (16- to 18-hour) pure culture was used as inoculation source. A single isolated colony was slightly streak on the surface of the simmon citrate agar slant. Incubate at 35ºC for 18 to 48 hours. Growth was observed at the slant surface and the color of medium was changed from intense green to a deep Prussian blue.