Next Generation Sequencing Analysis

Abstract

The DNA analysis becomes a cornerstone in contemporary forensic sciences. DNA sequencing technologies are powerful tools that enrich molecular sciences in the past based on Sanger sequencing and continue to glowing these sciences based on Next Generation Sequencing (NGS) technologies. NGS has impressive potential to flourish and increase the molecular applications in forensic sciences by jumping over the pitfalls of the conventional method of sequencing. The main advantages of NGS compared to conventional method are their higher throughput (parallel sequencing which will help in reducing DNA backlogs), lower cost and short run time with high resolution of genetic data. These advantages will help in solving several challenges that

Forensic DNA analysis continues to advance owing to development of new technologies. Addition of new informative markers to existing panel can improve discrimination power and accuracy. Next Generation Sequencing technologies (NGS) can bring new offers. Firstly, higher throughput which help in reducing significant DNA backlog in forensic laboratories. Secondly, parallel analysis became possible with NGS which can greatly improve the throughput (one run to all markers) and discrimination power. Thirdly, the clonal aspect of NGS platforms can also offer great advancement such as greater sensitivity for mixture analyses which considered as challenging samples. Lastly, with decreasing the cost and miniaturized equipment, it will become possible to do whole genome sequencing on a bench-top DNA sequencer. That will revolutionize the field of forensic DNA analysis. Recently, the impressive advances of sequencing technologies have enabled a lot of new applications. Therefore, this review will discuss the applications of NGS in forensic sciences as a simplified guide for future forensics studies and

Although there is a significant progress in solving some technical challenges associated with forensic STR profiling. Even with these technical advances, important challenges remain for the use of genetic approaches in human forensic investigations (Kayser and de Knijff 2011). Traditional capillary electrophoresis (CE) based STR typing rely on the detection of DNA fragment size. Therefore, alleles of similar length but of different sequences cannot be distinguished. Consequently, STR mutations in complex paternity cases often cannot be resolved with traditional CE-based STR analysis (Yang, Xie et al. 2014). An additional challenge for forensic DNA tests is the analysis of complex DNA mixtures comprising DNA from more than one person. Contemporary analyses of mixed DNA samples often yield low detection rates (Hu, Cong et al.