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Disadvantages Of Indirect ELISA

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Indirect ELISA
The term "indirect ELISA" refers to an ELISA in which the antigen is bound by the primary antibody, which then is detected by an enzyme labeled secondary antibody. The Secondary antibodies were raised against the primary antibody in other species of animal by immunization. The indirect ELISA has advantages over the direct ELISA.
1) The Indirect ELISA consist mainly two antigen – antibody reactions; the first reaction is between coated antigen and primary antibody and a second reaction between the primary antibody and the enzyme-labeled secondary antibody (with reference to secondary antibody the primary antibody is an antigen). Therefore, when the secondary antibody is enzyme tagged instead of primary antibody the amplified …show more content…

The sandwich ELISA has the ability to detect both antigen and antibody. The sandwich ELISA is near about same as that of the Indirect ELISA. In sandwich ELISA the antigen actively captured by antibodies, which are coated on the micro titer well plate.
The advantages of the ELISA are specificity, sensitivity, and safety. ELISA can detect a protein even in nanogram & picogram quantities. This test can be performed in various forms and is easily reproducible, standardized, and validated. 1.10 Species-specific antibodies for venom …show more content…

All methods use a reducing agent to convert ionic gold into metallic gold in a controlled manner. The most commonly used method is the sodium citrate procedure developed by Frens. In this method, the ionic gold solution was heated to boil and rapid addition of sodium citrate solution, immediately, ionic gold start to convert in metallic gold atoms and their concentration rises rapidly until the solution reaches

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