Although, in our lab report, suspect ones DNA matched the crime scene when cut with enzyme one, this can be explained by how closely related the two suspects are. Therefore two enzymes were used to cut the DNA; the suspect has to match both. Moreover, the limitation to DNA fingerprinting is, if a person were to have an identical twin. This is because identical twins have the same DNA because they come from the same egg. If a suspect’s DNA matched that of the one being tested, and they had an identical twin, a farther investigation would need to be done.
Introduction On October 19th 1992 witnesses believed they saw twenty three year old Laura Houghteling leave her Bethesda home, not knowing in fact that it was Hadden Clark, a part time gardner who worked for the Houghteling family. What would follow this sighting, and the subsequent missing persons report of Laura Houghteling would be a fascinating murder investigation which would lead to a precedent setting conviction and the capture of a serial killer. This report will investigate the method of Mitochondrial DNA analysis through the DNA typing method of RFLP or Restriction Fragment Length Polymorphism. The report will explore the mechanisms of the technique itself along with the benefits and limitations of the technique. This case is
In this three-week long experiment conducted in the Bio 13 Lab, we were able to analyze a single nucleotide polymorphism (SNP) in our own genomic DNA and then determine our genotype at this specific SNP. In week one, we extracted genomic DNA from our cheek cells with swabs and prepared our DNA for PCR (Polymerase Chain Reaction) that would amplify the region with the intended SNP of interest. After one week and after the PCR was run outside of the lab section, the resulting PCR product was purified and treated with restriction enzyme Ahdl in order to prepare for the final analysis of our genotypes. In the third and final week of the project, we analyzed our PCR products by means of agarose gel electrophoresis. By the conclusion of the experiment, we had completed the analysis at the SNP of interest and determined our genotypes for this SNP.
The STR length contrast is what is used to differentiate individuals. Gel electrophoresis then uses the STRs to create a DNA profile. The gel electrophoresis separates the STRs depending on their length and the pattern is then shown in fluorescent gel creating the profile. These profiles are then used by scientist to compare patterns between evidence and or suspects to determine a match or not a match.
He put forward about the integration approach. Integration
Sickle Cell Anemia a Negative and Positive Taylor Martin University of Missouri-Columbia September 23, 2015 Sickle Cell Anemia a Negative and Positive General Purpose: To inform my audience about Sickle Cell Anemia. Specific Purpose: As a result of my speech, the audience will be informed about Sickle Cell Anemia and how it can affect people. Central Idea: Sickle Cell Anemia has some negatives but, it can also be a positive in certain areas with the malaria virus. Introduction In America 70,000 to with sickle cell trait Sickle cell disease is an inherited disorder that affects red blood cells.
DNA in Forensic Science DNA is the carrier of genetic information in humans and other living organisms. It has become a very useful tool in forensic science since it was discovered. In forensic science, DNA testing is used to compare the genetic structure of two individuals to establish whether there is a genetic relationship between them. One example of the use of DNA in forensic science that is important in biology today is comparing a suspect’s DNA profile to DNA that was discovered at a crime scene.
Rh antigen is also present on the surface of RBCs similar to A, B and O antigens. Test for Rh blood grouping can be performed easily by side agglutination test. This blood group could be the most complex one of all blood type systems since it involves 45 different antigens on the surface of red cells that are controlled by 2 closely linked genes on chromosome 1. The inheritance of this trait can easily be predicted by knowing the simple genetic concept that the homozygous dominant i.e. DD and heterozygous i.e. Dd are Rh +ve and homozygous recessive i.e. are Rh