Dna Extraction Lab Report
In day one of weekend three the next experiment involved DNA extraction with the use of GeneElute Genomic Miniprep Kit-Sigma. Two heating blocks were prepared at fifty-five degrees Celsius and the other was prepared at thirty-seven degrees Celsius. Reagents were mixed with maximum effort and then examined for any sign of precipitation. If a precipitate formed then the mix was warmed to around fifty-five to sixty-five degrees Celsius until the precipitate dissolved thoroughly. It was cooled to room temperature before use. A pipette of 1.5 microliters of the overnight bacteria culture was labeled A and the tube was then transferred to a micro centrifuge tube. The overnight bacteria were labeled B and put into a second micro centrifuge. The cells
Unknown Culture Lab Report
1054 Words | 5 PagesIdentification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
Lab Report Identifying Unknown Microorganisms
1596 Words | 7 PagesIn this experiment, we cultivated an unknown specimen containing two microorganisms. The purpose of this experiment was to use a variety of biochemical test previously learned in the lab to identify the unknown bacteria. The identification of unknown bacteria is a major part of microbiology. Microbiologist observe samples such as blood and sputum in the laboratory for the presence of microorganisms. Identifying unknown bacteria is extremely important in clinical settings because it helps physicians find treatment for infections.
Unknown Bacteria Essay
498 Words | 2 PagesAfter incubation, a gram stain was performed one the colonies that were isolated. First, the organism was smeared onto a slide with a loop full of distilled water. The smear was heat fixed to provide the bacteria to stick to surface. Next, the staining started by using crystal violet for 60 seconds, rinsed with distilled water. Then iodine for 60 seconds, rinsed with
Unknown Bacteria
557 Words | 3 PagesIn the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
Recombinant Dna Technology Lab Report
681 Words | 3 PagesThough, locating a specific gene within the DNA sample can be extremely difficult. There are roughly 6 feet of DNA, consequently a small tissue section will comprise countless kilometres of DNA. Recombinant DNA technology has caused it to be achievable to separate one gene or any further sections of DNA. This
Dna Fingerprinting Lab Report
1726 Words | 7 PagesDNA Fingerprinting Using Agarose Gel S. Aaron Sowards Bio 122 Lab 04 Brianna Adanitsch Jakob Lester Minhenga Ngijoi 2/21/18 Dr. Chad R. Sethman Abstract DNA fingerprinting is the process of analyzing an individual’s DNA base-pair patterns. The DNA fingerprinting lab involved identifying the suspect using Agarose Gel and Polymerase Chain Reaction. It was found that suspect two s DNA matched the crime scene DNA.
Dna Fingerprinting Lab Report
2151 Words | 9 PagesThe lab focuses on DNA fingerprinting which is usually done in forensics to catch criminals by the analysis of DNA fragments of different sizes by a method called electrophoresis. Electrophoresis is a separation technique that uses an electric field to separate and distinguish biological molecules (this including DNA) in the experiment, gel is used to as a way to apply friction in electrophoresis which can help you easily distinguish between the DNA because of different sizes in the DNA confirmations. The goal of the experiment was to identify between two separate suspects DNA with restriction enzymes that help tell apart the suspects and who was actually at the crime scene. The methods used were the use of a gel made from agarose placed in a gel tray connected to an electrical current because of the DNA being negative and being able to be pulled apart to the positive side and therefore getting a clear spread of the DNA that was cut with restriction enzymes. The conclusion of this experiment was that suspect two was the criminal because of the crime scene DNA that was found matched the DNA of the second suspect when cut with
Polymerase Chain Reaction Essay
1132 Words | 5 PagesThe final stage of PCR is extending stage when the temperature is raised to 72 degrees Celsius and a new strand of DNA is made by an enzyme called Taq DNA polymerase which is taken from the heat-loving bacteria Thermus aquaticus. This three-thermal process is repeated 20-40 times to produce many copies of the interested DNA sequences. (Hughes and Moody, 2007)
Forensic DNA Analysis
1512 Words | 7 PagesIn the United States, the death penalty and the question of executing innocent people has become a fundamental topic of discussion. Jay D. Aronson and Simon A. Cole propose that, “due to the certainty attached to DNA evidence in public discourse, it can be used as a lever with which to challenge law’s claims to truth-making authority, and to undermine public trust in the death penalty” (Aronson and Cole 603). Shlomit Avraham maintains that “the success of obtaining DNA profiles from touch DNA has opened up possibilities and led to the collection of DNA from a wider range of exhibits” (Avraham 793). How many people have been released or imprisoned due to faulty accusations? Where are DNA samples found, and what is it?
Isolated Genes
691 Words | 3 PagesThe pellet was washed by slowly adding 500 μl of 70% ethanol before being centrifuged for 2 minutes. The supertanent was removed and the DNA pellet was allowed to dry for 5 minutes. The pellet was resuspended in 200 μl of DNA Resuspension Buff by vortexing. The samples were then placed in ice to
Mitosis Lab Report
1407 Words | 6 PagesEukaryotic cells divide and reproduce in two ways: mitosis and meiosis. Mitosis is a process of nuclear division that chromosomes are separated into two identical sets of chromosomes. The purpose of mitosis is cell regeneration, growth, and asexual reproduction. Meiosis, on the other hand, is a special type of cell division which reduces the chromosome number by half. To achieve halving the genome, DNA replication is followed by two consecutive rounds of nuclear division during meiosis.
Multiplex Polymerase Chain Reaction Lab Report
1902 Words | 8 PagesMultiplex Polymerase Chain Reaction The development of the Polymerase Chain Reaction (PCR) has allowed for both the rapid and efficient amplification and analysis of specific DNA sequences. Generally speaking, PCR is specifically designed and performed to amplify one target sequence using only one set of oligonucleotide primers. However, there are several different experimental approaches that require multiple DNA sequences to be analysed. Using the ordinary PCR method, this requires that multiple PCRs be performed on the same or related DNA templates, which would prove to be very time consuming. This is where a process known as Multiplex PCR comes into play.
Mitosis Lab Report
638 Words | 3 PagesBefore I can go into detail about the three steps of mitosis that are presented in the question I must first summarize mitosis. Mitosis is actually a process of nuclear division that is nicely broken up into five stages. The stages are prophase, prometaphase, metaphase, anaphase, and telophase. Mitosis conserves the chromosome number by assigning replicated chromosomes equally to each of the daughter nuclei. Mitosis takes place in eukaryotic cells (Campbell, pg. G-22).
Biochemical Test Lab Report
1371 Words | 6 PagesBiochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
Meiosis Lab Report
1004 Words | 5 PagesMeiosis Introduction Meiosis is a special type of cell division in which the number of chromosomes in daughter cells is reduced to half, as compared to the parent cell. It takes place in diploid cells only, in animals at the time of gamete production while in plants when spores are produced .There are two meiotic divisions. The first meiotic division is the reduction division whereas the second meiotic division is just like mitosis . Meiosis I
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