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Dna Isolation Lab Report

2367 Words10 Pages

Isolation, Digestion, Visualization of DNA and Analysis of Its Structure, Transcription and Translation

By Canyu Yu (Celine)
1004145702

IFP 070
April 2, 2018

Lab Partners: Victoria Zhu

Introduction:
DNA?short for Deoxyribonucleic Acid), also known as deoxyribonucleotide, is a major component of chromosomes and a major genetic material. It can form genetic instructions to guide biological development and vital functioning (Irobalieva et al., 2015). All living organisms on the earth need for DNA in order to pass along their genetic material to offspring. In the process of DNA isolation, high concentration of ethanol will be added to form DNA …show more content…

Through enzyme digestion experiments, I am able to understand the role of restriction enzymes in bioengineering. And I can clearly see the length of the fragment when the restriction enzyme cleaves DNA. This experiment also made me gain knowledge about DNA analysis. Through the experiment of Restriction Endonuclease Digestion of DNA, I learnt that when using RedSafe stain and the UV light source, I should be careful because their mutagenic effects. Moreover, this experiment makes me understand the role of gel electrophoresis in DNA preparation and analysis. Through the experiment of building DNA, I knew more about the composition of DNA, and the way and order of deoxyribonucleotides. Through the DNA transcription and translation experiments, I learnt that mRNA is the transmitter of genetic information. I also knew that a genetic code corresponds to an amino acid. This made me have a deeper understanding of the process from genetic information to protein. Overall, I learnt that DNA is insoluble in ethanol; lambda phage DNA was digested by Hind III and EcoR I in the Sample 3E and was digested by Hind III in the Sample 4E. DNA can be visualized by agarose gel electrophoresis, and the direction of DNA synthesis is from carbon 5 to carbon

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