12. CHAPTER 7
12.1. DNA DAMAGE
12.1.1. INTRODUCTION
Toxic metal pollutants in marine water tend to increase steadily thereby representing the greatest hazard to aquatic organism. Investigations on the toxic effect of metals upon bivalves is accompanied by the analysis of changes in nucleic acid indices (Hoyle et al., 2007). Comet assay, as method for detecting DNA alterations and micronucleus test, as an index of chromosomal damage are the most widely applied for genotoxicity biomarkers.A number of studies were carried out to evaluate therole of oxyradical metabolism in inducing genotoxicdamage associated with different pollution gradients.Several biomarkers, including DNA integrity, damage, oxidized bases, oxyradical metabolism and the impairment of lysosomal membrane stability, were appliedin haemocytes of mussels Mytilus galloprovincialiscaged for four weeks in Genova harbour IT. Organismswere collected at different time intervals to
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The fluctuations of DNA strand breakswere analysed in mussels Mytilus galloprovincialis,seasonally sampled over a three year period from a reference site along the Adriatic coast, showing changes inDNA damage extent in haemocytes, with marked differences between various sampling years (Pisanelli et al.,2009).A biomonitoring study was carried out in KaštelaBay and the neighbouring Trogir Bay, Croatia, using themicronucleus test and Comet assay in haemocytes fromnative and caged mussels Mytilus galloprovincialis. Theresults show increased DNA damage along a pollutiongradient with higher levels in caged mussels than innative ones. (Klobucar et
The design relied on two Schmitt triggers to generate the two different tones while using the transistors to act as a switch. This causes it to trigger continuously between two unstable states, allowing automatic switching between two frequencies producing two different tones. The RC values between the two Schmitt triggers will differ. Capacitors charge and discharge faster when it’s resistance is smaller.
Discussion 1. Zn0 (s)+ Cu2+S6+O42-(aq) →Cu0(s) + Zn2+S6+O42-(aq) Zn0(s) → Zn2+(aq) + 2e- Cu2+(aq) + 2e- → Cu0(s) Zn0(s) + Cu2+(aq) → Zn2+(aq) + Cu0(s) Oxidant (oxidizing agent) is the element which reduces in experiment.
Cadet Eric Wiggins Date: 18 September 2014 Course Name: Chem 100 Instructor: Captain Zuniga Section: M3A Identification of a Copper Mineral Intro Minerals are elements or compounds that are created in the Earth by geological processes. The method of isolating metals in a compound mineral is normally conducted through two processes.
Suppose you need to find the fractional European call and the fractional European put options. Let the Hurst parameter be $H=0.85$, the $\sigma=0,25$, $r=0.10$, $S_{fbm} = 100$, $K = 95$, we have \begin{eqnarray*} d_1^{fBm} & = & \frac{\ln{\frac{S}{K}} + \frac{1}{2}(r( T - t) + \frac{(1)\sigma^2{( T^{2H} - t^{2H})}}{2})}{\sigma{\sqrt{T^{2H} - t^{2H}}}}\\ & = & \frac{\ln(\frac{105}{100}) + (0.10(0.25 -0) + \frac{(1){0.25^2}{0.25^{2(0.85)} - (1)0.25^{2(0.85)}}}{2}}{(0.25){\sqrt{0.25^{2(0.85)} - 0}})} \end{eqnarray*} we obtain $d^{fBm}_1= 1.0558$. We find in the normal distribution that $N(1.0558)= 0.8544$ and $N(-1.0558) = 0.1456.$
Testing phase finds differences in positive/negative documents by the centroid obtained in training phase by ranking each of them. The simple way to estimate similarity between documents and centroid by summing weights of patterns which are in the documents. VII. Experimental Results To determine accurate measures of similarity or difference between documents you depict results by graph pattern and table pattern. The experimental setup consists of relevant documents that you termed as positive and negative documents .i.e
1. Identify the range of senses involved in communication • Sight (visual communication), Touch (tactile communication), Taste, Hearing (auditory communication), Smell (olfactory communication) 2. Identify the limited range of wavelengths and named parts of the electromagnetic spectrum detected by humans and compare this range with those of THREE other named vertebrates and TWO named invertebrates. Figure 1: the electromagnetic spectrum source: www.ces.fau.edu Vertebrates Human Japanese Dace Fish Rattlesnake Zebra Finch Part of electromagnetic spectrum detected ROYGBV (visible light) detected by light sensitive cells in the eye called rods and cones.
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
Background: In Ischia Island the water around the island has been acidified for millions of years because of Mt. Vesuvius. This dormant volcano is melting chalk in the sea floor, which releases carbon dioxide into the water acidifying it. This acidification is devastating the life around the island and is still being acidified by the continuous release of CO2. We can use this as an example of what our world might look like if we continue to release carbon dioxide emissions through things such as burning fossil fuels and reducing the carbon banks such by things like deforestation.
Tn 4351 was originally isolated from bacteroides fragilis [30] . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation [25]. Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides.
2.4 Band Division and Energy Computation: The power spectrum of the signal is multiplied by magnitude response of set of 33 triangular band pass filters and in the range 300Hz-2000Hz. Sub-bands are formed by using the logarithmic spacing. The positions of these filters are equally spaced along the Mel frequency, which is related to the common linear frequency f by following formula: Mel (f) = 1125* ln (1+f/700) (3) Mel frequency is proportional to the logarithm of linear frequency and which is close to the human perceptual system. 2.5 Sub Fingerprint Generation:
• Write down the highlighted numbers. Do you observe a pattern? • Does the pattern grow? What is the reason for this? • Write down the last number (say 53).
Elijah Brycth B. Jarlos IX-Argon 1. Multicellularity is a condition of an organism to have multicellular cells. An example of a organism who has multicellular cells are plants, animals, and humans. The main reason of why scientists have a hard time finding a good set of existing organisms to compare. Is neither the first set of organisms which is being compared is dying as fast as the second specimen is being examined or they just can’t find the right species.
Water Pollution is a huge environmental problem that has serious effects on marine habitats, animals, and water quality. BP1: The world's marine habitat has been getting worse because of how bad the pollution has gotten. “ Over 80% of marine pollution comes from land based activities “ (source 5 ). This quote explains how most of the water pollution is caused by human activity. “ Plastic litter does not degrade, can be expected to last in the oceans for hundreds of years “ ( source 6 ).
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
Marine pollution such as presence of organic matter in the ocean can result in the condition known as hypoxia or oxygen depletion and this can have adverse effect on the marine life including plant and animals and fish. Death of these fish can result in loss of millions of US dollars that are generate from the fishing industry. Marine pollution can also result in presence of foul smell resulting from the decomposing sewage being directed to the ocean and this has the effects to hinder recreational activities taking place in the sea as well as cause discomfort and breathing problem to the surrounding population (Laura, 11).marine pollution can also result in danger to human health. The human swimmers and water sport lovers can become endangered by swimming in the polluted marine waters (Laura,