Dna Synthesis Lab Report

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Abstract: Molecular analysis of DNA encompasses a series of separation, amplification and detection techniques that are used to determine the source of origin of an organism’s tissue sample. It correlates genes’ sequences with their functions, and allows the identification of the unknown organism. This study was done to see whether the techniques of molecular genetics like extraction and polymerase chain reaction could be used to find the animal whose tissue were sampled. GENEIOUS software was used to analyze and align the electropherograms results before GenBank and BLAST were used to identify the unknown DNA sequence by comparing it to a set of already known sequences. The results indicated that the better the fragmentation of the DNA sequences were in the PCR, the better it would be assayed by electrophoresis and the more samples could be used in the CSR; thus, the more accurate the sequences would be. This would help to identify the unknown meat’s animal of origin better. Introduction: Deoxyribonucleic acid, DNA, is the genetic material of all living organisms, and it is found in the eukaryotic cells’ nuclei. It could be extraction from an unknown tissue sample, assayed and compared to already known sequences in order to find the organism’s source of origin. Molecular analysis of DNA encompasses a series of…show more content…
However, unlike in PCR, one primer is used instead in CSR, so linear amplifications of the products are made. Dideoxyribonucleoside triphosphate, ddNTP, terminators are fluorescently labeled and a laser within a sequencing machine is used to analyze the produced DNA fragments by relaying information and generating an electropherogram of the sequence. DdNTPs are also used to terminate the chain growths (Fan et al.,

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