Embedding was done with two changes of molten paraffin for 30 min to remove xylene. They were mounted in L blocks with molten paraffin and solidified. The solidified blocks were trimmed to small size and sectioned using microtome (Spencer U.S.A) 3 to 5-micron thickness. 5.2.3 Toluidine blue Staining: Toluidine blue is a metachromatic stain that stains nucleus which appears blue in color. The isolated rat's brain was fixed and processed as per the tissue processing procedure, and then brain tissue slices were prepared for toluidine blue staining.
Repeat step four for each sample with a new sterile swab each time. 6. Label the petri dishes according to their samples, and seal each with tape. 7. Then, to take data, each day place the 0.5cm^2 piece of grid paper underneath the petri dish and count the approximate number of bacteria in one
Lab-experiment immunity and bacteria- How do they react? Research question: How does the bacteria Enterococcus Faecium SF 68 demonstrate resistance against the following antibiotics: Oxacillin, Climdacylin, Penicillin-G, Amikacin, Lincocymin, Erythromycin, Cephazolin, Mezlocillin ? Terminology used: Bacterium: Singular form of bacteria, one single individual. A bacterium an organism that possesses one single cell and is very adaptable to most environments. A bacterium contains only a single chromosome, but posses more sections of DNA known as plasmids, that are spreading all around the bacteria in an area called the cytoplasm.
Next, place the container of breast milk into the refrigerator, which will allow it to slowly thaw over the next 12 hours. Milk thawed in the refrigerator must be used within 24 hours. Thawing Breast Milk Do 's and Don 'ts Do Freeze Your Breast Milk in Small
We took 2 Petri plates containing L-agar and spread 50µl from dilutions 〖10〗^(-3) and 〖10〗^(-5) respectively on L-agar plates by using spreader and incubated them at 37°C for 24 hours. After 24 hours incubation, we selected isolated colony and streak it on L-agar containingPetri plates to purify it. After 24 hours incubation, we got our purified colony, now we checked either it is nitrogen fixing bacteria or not, for this purpose we performed “Nitrite Detection Test”. Nitrite Detection Test: We made mother culture of our strain. We took 3 test tubes and added 3 ml ammonium sulphate broth and autoclaved them.
However, when these bacteria are grouped together to have high cell density, the molecules they secrete amount to a certain number, and once that number is reached, the behavior of that bacteria is switched on and in this case, bioluminescence is created. Similarly, in my project, I am screening the anti bacterial activity using oils. Before I use the oil, I have to culture the bacteria overnight so that I could use them in the plates after 16-18 hours of incubation. Based on the talk, I believe that I have an idea on how bacteria grow. This Ted Talk has inspired me about science in numerous aspects.
Using a disposable plastic pipet, worms were transferred with a bit of spring water to the viewing chamber and given a few minutes to settle to their new surroundings. The viewing chamber was then placed under the dissecting microscope at the lowest power, which helped with focusing on the middle body region of the worm to measure pulsation rates. Using a stopwatch, the basal rate of the worm was obtained by counting the number of pulses that moved through a segment in a thirty second interval, this amount was multiplied by two to result in units of beats per minute. Three basal rates were recorded for each of the three individuals warms to calculate their mean rate. Worms A, B and C were then placed into separate containers containing the caffeine treatment solution.
Store the petri dish in the incubator Data: Day 1 Dish was spotted with slight growth of bacteria. Each section had a different type/number of bacteria: the table was a variety, the keyboard was all the same yellow dots, the cellphone has a big brown dot, and the bottle had a couple of yellow
The swab was inoculated onto blood agar, MacConkey’s agar and nutrient agar, mannitol salt agar and Sabouraud's dextrose agar and was incubated at 370C for 24 hrs for bacterial culture and at room temperature for fungal isolates. Next day the colonies were picked up and preliminary identification was done and the bacterial isolates were identified based on standard protocols. LPCB was done for the identification of fungal isolates after 48 to 72 hrs. ANTIBIOTIC SUSCEPTABILITY TEST Antibiotic sensitivity test of the bacterial isolates was determined by the Kirby-Bauer disc diffusion method2. The following were the antibiotics used for the study-amikacin (AK 30), nalidic acid (NA 30), erythromycin (E 15), vancomycin (VA 30), tetracycline (TE 30), cefoxitin (CX 30), rifampicin (RIF 5) ciproflaxicine (Cip 5), ceftazidime (CAZ 30), cefotaxime (CTX 30), cepifime (Cpm 30), cefoperazone (CPZ 75).
Those which aggregated with salt particles and formed clumps were considered hydrophobic. E.coli grown on MacConkey agar plates were inoculated into 1 ml of Phosphate buffer saline (PBS) at pH 6.8 and turbidity was matched with McFarland tubes 6 or 7 to get a colony count of 5x109 colonies/ ml. Different molar concentrations of ammonium sulphate namely 0.625M, 1.25M and 2.5M were prepared. One loopful (10µl) of bacterial suspension made in PBS was mixed with equal volume of ammonium sulphate solutions of different molarity on a clean glass slide and observed for one minute while rotating. The highest dilution of ammonium sulphate solution giving visible clumping of bacteria was noted as the salt aggregation test value.