E. Coli Bacteria

Water has a huge impact on human health and should be tested for fecal coliforms to maintain safety for the human population. Lake Byron, one of the water samples used in this experiment, will contain the most fecal coliforms because the surface of the water temperature is constantly rising to be ideal for growth of bacteria. The amount of positive Durham lactose broth tubes helped us to identify the MPN/100 mL of fecal coliforms. Inoculating an EMB plate with a positive coliform helped us suspect the possibility of E. Coli, and we were able to complete an IMViC series to identify the bacteria found in the water sample. Our results indicated that a water sample from USM Creek had the highest number of fecal coliforms, and a swimming pool water

This report is about identifying the respective genus of the given unknown organism. The goal is to show and prove the student’s understanding of microbiology and laboratory learned experimental techniques. The student is to use studied tests to eliminate possible generas and to isolate the correct genus and identify it to the given unknown. This proves the diversity of microorganisms and their uniquely varying traits. The resulting data obtained in class will enhance students’ laboratory skills and knowledge regarding microbial laboratory.

Aseptic technique was initiated at the beginning of this experiment by cleaning the work surface with disinfected wipes. Personal protectives equipment was also worn. The material utilized in this experiment was: S. epidermidis culture broth, sterile cotton swab, streak plate, forceps in 70% alcohol, a lit tea light, and the three antibiotic disks (novobiocin, gentamicin, penicillin). The first step, I divided a plate into three quadrants and labelled them with the different antibiotic names. Using the lit tea light, like a bursen burner, I flamed the mouth of the S. epidermidis culture. The sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used. The plate was incubate with lid up on the bookshelf at room temperature for 48 hours. After 48 hours, I observed different growth patterns around the disks. I measured the zone of inhibition of each antibiotic and document them on Microbiology task 3

The table shows that a gram stain, and endospore were the only test performed to the unknown bacteria “W”.

The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not

Genetic engineering is changing the DNA code to express different traits. A plasmid is a circular piece of DNA that contains important genetic information. Recombinant DNA is the product after inserting your desired genes. The genes we hoped to insert in the pGLO lab were the GFP gene and the ampicillin resistance gene. GFP was needed so that we would tell if the ampicillin resistance gene had been properly placed when the bacteria glowed under a UV light. The purpose of this lab was to perform a procedure known as genetic transformation which allowed us to genetically engineer E. Coli to be ampicillin resistance.

The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media.

Introduction: Transforming a gene or genetic information from one organism into another with the hopes that if done successfully the organism with the new DNA will be given new traits is a method known as genetic transformation (Rafter). Genetic transformation is used quite frequently in today’s world, form medicine to agriculture.

Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398.

In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates. Each plate serves as a first step to identify the unknowns. The TSA (tryptic soy agar) can be used to do a gram stain, which differentiates gram-negatives from gram-positives, based on the structural make up of the cell wall (Carson, 2015). The blood agar plate is used to test for hemolytic activity, which is useful for distinguishing gram-positives. A MacConkey plate is selective by inhibiting the growth of gram-positives and differential due to the fermentation of lactose by certain gram-negative species.

In the book Missing Microbes, the author, Dr. Martin J. Blaser discusses different types where the mysterious microbes are to be found. Dr. Martin also discusses his hypothesis in which talks about how over use of antibiotics has permanently changed the microbiome that humans live in, causing an increase in more modern diseases. The way Blaser lays the book is more like a journey; he traces his footsteps, and has the readers following the lead anxiously waiting on what he will inform them. There are a lot of doors in Science. Dr. Blaser chose to enter the door where facts and stories are to be learned everyday, in which there is no end, making that the beauty of science. Dr. Blaser starts the book by describing how much humans have been using antibiotics, describing the use as more of an addiction. Dr. Blaser talks about how individuals use antibiotics in order for them to fight against bacterial infections. Also discusses using antibiotics as an agricultural input for industrial farming operations. In the text, Dr. Martin J. Blaser does argue against antibiotics. Dr. Martin Claims the reason in a clear thoughtful

Our predictions were that for the standard protocol plates with agar and ampicillin, there would be growth and the colonies would glow under UV light. On the modified protocol on plates with agar and ampicillin where we changed the time of the heat shock, we expected less growth but the colonies would still glow. On the first negative control plate, there should have been no growth on the plate with agar and ampicillin and regular E. coli cells. On the negative control plate with just agar and E. coli cells there should have been growth but the colonies would not

Strains of E. coil are important in the digestive tract, but others may cause problems in urinary and intestinal tracts. Certain types of E. coli strains show resistance to bacteria which kills antibiotics. The resistance is because of the plasmids. Resistance plasmids are broadly studied and bestow resistance to factors which may hinder growth of the organism. Resistance plasmids signs for proteins which will inactivate the antibiotic affect their reception into the bacteria (Weinreich, 2006). We deal with two different strains of E. coli in this lab i.e. Standard E. coli which lacks resistance plasmid and strain containing a resistance plasmid which has genes that are protecting it from

In conclusion carprofen has no significant effect on bacterial treatment. Carprofen was given late into the acute phase reaction of the induced E.coli when clinical signs were first seen. Treatments with carprofen had modulatory effects on clinical and immunological parameters. Results show that after a treatment with carprofen, there was a significant decline in severity of the clinical parameters. Carprofen is a great antipyretic but does not exhibit its major immunomodulatory effects that would have been expected.

The media used in this experiment was Trypticase nitrate broth. The reagents used (A and B) were sulfanilic acid and alpha-naphthylamine (respectively). Using aseptic technique, the bacterium (16A and 16B) were inoculated into labeled broth test tubes. The tubes were incubated for 48 hours at 37 degrees Celsius. When the incubation was complete 5 drops of reagent A and 5 drops of reagent B were added to each of the broths. If the broth turned a reddish color, the result was then positive. If there was no color change, then a small amount of zinc powder was added. If there was no color change, the result was also positive, but if there was a red coloration development after the zinc was added, the result was then negative. Both Unknown bacteria (16A and 16B) were positive for nitrate reduction. The tubes were then