Eastmamol/540 Week 4
Prelab week 1 Calculations Preparation of 1.5μmol/L mixed low-level standard dilution 150μmol/L × V1=1.5μmol/L × 10ml V1=(1.5μmol/L×10ml)/(150μmol/L)=0.1ml Conversion of milliliters to microliters (0.1ml×1000)μL= 100μL Preparation of 3μmol/L mixed low-level standard dilution 150μmol/L × V1=3μmol/L × 10ml V1=(3μmol/L×10ml)/(150μmol/L)=0.2ml Conversion of milliliters to microliters (0.2ml×1000)μL= 200μL Preparation of 3μmol/L mixed low-level standard dilution 150μmol/L × V1=7.5μmol/L × 10ml V1=(7.5μmol/L×10ml)/(150μmol/L)=0.5ml Conversion of milliliters to microliters (0.5ml×1000)μL= 500μL Preparation of the blank samples The volumetric flask will be filled to the mark with 150μmole/L of stock solution to act as blank (reference). Additional two blanks will …show more content…
2010). RP-HPLC is recognized by a condition in which the mobile phase is made to be more polar as compared to the stationary phase. In reversed HPLC, the stationary phase is hydrophobic. It is also chemically bonded to the surface of silica support elements. The reversed HPLC mobile phase for neutral analytes is composed of organic modifier and water. Organic modifiers are used to change the retention time of different analytes. Organic modifiers lower mobile phase polarity. By increasing the amount of water lead to the repulsion of hydrophobic analytes out of the mobile phase. The hydrophobic analytes are pushed onto the stationary phase where they reside for duration up to the partitioning into the mobile phase. When ionic analytes exist in the sample, the addition of ion and buffer to the mobile phase are necessary. This addition aids in controlling the reproducibility and retention. Separation of the mixture via RP-HPLC can be done using continuous gradient or stepwise to move out the sample components. For every separation, the ideal gradient and volume must be
6.03 Calorimetry Lab
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Acct 555 Week 1
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1545 Words | 7 PagesThe colour of each test tube was recorded and if proteins were present that was recorded for each test tube. Finally, the pH was recorded for each sample using pH
Percent Cu2 + In A Mineral Sample
931 Words | 4 PagesThen the flask was filled the rest of the way with distilled water to the mark. Similar steps were taken for the rock solution. The rock solution from the prior lab was filtered into a volumetric flask (100mL), then 15 M NH4¬OH (8mL) was added to the flask. After that, the flask was filled to the mark with distilled water. Both flasks were then swirled to combine the solution
Eco/372 Week 1
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Non Opioid Analgesic Essay
473 Words | 2 PagesThe drawback is that column chromatography is very time consuming; one way to combat this is to utilize flash chromatography, which involves a nitrogen pressure stream pushing the mobile phase through the column. The differences in polarity allow for the effective separation of the various components. The more polar compounds adhere to the polar silica or alumina stationary phase for a longer time. The less polar components elute first and then the polarity of the solvent is increased in order to elute the more polar compounds. Collecting small fractions is essential in column chromatography because they can be combined together; large fractions can lead to multiple compounds in a specific fraction.
Comparing The Calculation Of Kool-Aid Tropical Punch And Strawberry
192 Words | 1 PagesThen, 5 mL of the halved concentrated solution was measured and added into another volumetric flask. 5 mL of deionized water was added to produce a solution that was a fourth of
O Toluidine Vs Hexane
184 Words | 1 PagesBased on the data, the eluent system that represented the best separation of the three compounds, anisole, benzoic acid, and o-toluidine, was the 4:1 hexanes to ethyl acetate ratio because it showed the greatest distance between the compounds. The highest Rf values indicated that the least polar compounds, which traveled the furthest distance, were least attracted to the polar plate due to the smaller amounts of hydrogen bonding that occurred between the compound and the plate. Anisole had the highest Rf values, of 0.920 and 0.773, compared to benzoic acid and o-toluidine because it had the least hydrogen bonding ability to the silica gel, while o-toluidine and benzoic acid may have been able to form more hydrogen bonds to the polar gel. O-toluidine
Describe The Procedure And Explain How They Influence The Purity Of
423 Words | 2 Pages1. How pure is your sample? When analysing our sample under UV light we could see if our sample was pure. We labelled the sample with 1= which was our sample, 2= the pure aspirin sample, 3= the salicylic acid.
Mat 540 Final Paper
801 Words | 4 Pages= 10^-3 M = 1,000 mL Here C1,C2; are the first and second concentrations of solution V1 and V2 ; are the required and current volumes. The impeller turned on and DDA, and tap water left to be mixed properly with water for 2 minutes. Approximately 150 grams of quartz added into the solution.
Withania Somnifera Report
876 Words | 4 PagesAdd another 25cm3 of Methanol and Ethyl acetate to the solutions. Stir gently for 20 minutes using a stirring rod this is to allow more of the active ingredients to mix with the solution. 16. Take two funnels and place one in two separate clean measuring beakers making sure the bottoms of the funnels don’t touch the bottom of the measuring beakers. Take two pieces of filter paper and press one onto each funnel.
Dieldrin Heptachlorimetric Analysis
1206 Words | 5 PagesThe lipid-adjusted concentration of an analyte was given by C lipid adjusted = [CONC/TL] ×
Lab Report Spectrophotometry Lab
851 Words | 4 Pages7) Test tube four contains 7 mL of distilled water and 3 mL of CoCl2. The fifth had 7 mL of distilled water and 3 mL of CoCl2. 8) Test tube five contains 6 mL of distilled water and 4 mL of CoCl2. 9) Test tube six contains 5 mL of distilled water and 5 mL of CoCl2. 10) After all of the test tubes are prepared, they will be put into cuvettes.
Cycloodiline Lab Report
1066 Words | 5 PagesAgar dilution factor must be allowed in the first calculation as follows: • The final volume of the medium in the plate equals 20ml • Top concentration of the antibiotic equals 64mgL • Drug total amount equals 1280 microgram is added to 1ml water • 2mls of 1280microgram per ml is required to start the dilution equals 2560micrograms in 2mls. • 1.28mls of 2000micrograms per ml ± 0.72ml of water.
Nucleotides Lab Report
972 Words | 4 PagesIon-paired reverse phase liquid chromatography for the detection, separation and quantification of nucleotides If you are working in the field of molecular biology, there is hardly a day that goes by without the use of nucleotides. But beyond the use of the four well known deoxynucleotides in PCR, there are several other uses of nucleotides. In the field of enzymology, nucleotides are used as substrates of various enzymes. For example, kinases and phosphatases use nucleotides as substrates while phosphotransferases transfer phosphate group from one nucleotide substrate to another. If you want to study the kinetics of such enzymes, you need to quantify either the substrate or the product.
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