Electron Microscope Essay

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Antony van Leeuwenhoek (1632-173) was responsible for on of the earliest developments of the microscope, it consisted of a powerful convex lens and an adjustable holder for the object being studied. This instrument would have been able to magnify objects up to 400x and with it he discovered protozoa, spermatozoa, and bacteria, and was able to classify red blood cells by shape. The limitation with this microscope was the single convex lens. This was fixed by the addition of a second lens, giving rise to the compound microscope which is the basis of light microscopes today.

During the 18th century, technology developed into using a beam of electrons for the microscope unit to improve resolution capabilities. The first electron microscope was built by Max Knoll and Ernst Ruska in 1931, this gave rise to a greater resolution than with light microscopy in 1933. The first electron microscopes were transmission electron microscopes (TEM) and they became commercially available in 1939. The development in this type of microscope unit aided in fields such as human biology, botany,
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It works by shooting a light emission at a thin cut of a specimen and distinguishing those electrons that endure to the opposite side. The TEM gives us a chance to look in high determination at a thin area of an example (and is along these lines similar to the compound light magnifying instrument). This makes it especially useful for finding out about how segments inside a cell, for example, organelles, are organized.

Electron tomography is a type of TEM that gives us a chance to see a three-dimensional perspective of the cell or tissue being examined. Seeing structures in three measurements can make it considerably less demanding to see how they identify with each other. Electron tomography can likewise give two-dimensional pictures at higher determination than routine

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