CHAPTER 4
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH PDA DETECTOR FOR COMBINED DETERMINATION OF EMTRICITABINE, TENOFOVIR AND EFAVIRENZ
The present work describes a simple assay method for determination of emtricitabine, tenofovir and efavirenz in their combined tablet dosage form by using HPLC with PDA detector. Our aim was to develop a simple, accurate, sensitive method for simultaneous determination of emtricitabine, tenofovir and efavirenz in combined pharmaceutical dosage form by HPLC with UV detection, where simple mobile phase composition was used for chromatographic separation without any ion-pairing agent. Total retention time for analysis was short with a good resolution between emtricitabine, tenofovir and efavirenz. All these
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The output signal was recorded on the monitor and then quantified by using Empower2 Software. The separation was done by Agilent Eclipse XDB column (length 150mm × 4.6 inner diameter, 3.5µ particle Size) with a guard …show more content…
The quantity of tablet powder equivalent to 200 mg of emtricitabine, 300 mg of tenofovir and 600 mg of efavirenz was transferred to 100 ml volumetric flask consists of 30 ml of mobile phase. The solution was sonicated for 20 mins and made up to the mark with the mobile phase and then filtered through 0.45 µm membrane filter. The resultant solution was diluted appropriately with the mobile phase to get a final concentration of 200 μg/ml emtricitabine, 300 μg/ml tenofovir and 600μg/ml efavirenz.
Results and Discussion
HPLC parameters
%% Init % clear all; close all; Fs = 4e3; Time = 40; NumSamp = Time * Fs; load Hd; x1 = 3.5*ecg(2700). ' ; % gen synth ECG signal y1 = sgolayfilt(kron(ones(1,ceil(NumSamp/2700)+1),x1),0,21); % repeat for NumSamp length and smooth n = 1:Time*Fs '; del = round(2700*rand(1)); % pick a random offset mhb = y1(n + del) '; %construct the ecg signal from some offset t = 1/
Suppose we have a single-hop RCS where there is one AF relay that amplifies the signal received from a transmitter and forwards it to a receiver. Assume that the transmitter sends over the transmitter-to-relay channel a data symbol ${s_k}$, from a set of finite modulation alphabet, $S={S_1, S_2,ldots,S_{cal A}}$, where ${cal A}$ denotes the size of the modulation alphabet. The discrete-time baseband equivalent signal received by the relay, $z_k$, at time $k$ is given by egin{equation} z_k = h_{1,k}s_k + n_{1,k},~~~~for~~k=1,2,ldots,M label{relaySignal} end{equation} where $n_{1,k}sim {cal N}_c(0,sigma_{n1}^2)$ is a circularly-symmetric complex Gaussian noise added by the transmitter-to-relay channel, $h_{1,k}$ denotes the transmitter-to-relay channel, and
1. The test subjects will prepare for sleep by acquiring everything needed for the subjects’ sleep preferences. 2. The test subjects will all set alarms on their smartphones for approximately 6, 8, and 10 hours after the subjects’ enter the resting period (Subjects may wake during the resting period for the bathroom, but they must not stay awake for more than ten minutes at a time to prevent as much deviation as possible.). 3.
Medical biller is a position that will require you to take in medical claims and code them and bill out medical claims to insurance companies, Medicare and Medicaid on a daily basis. You will have to reconcile Explanation of Benefits (EOB) weekly. Verify if insurance companies require that patients get PA for certain procedure and products. Five requirements for Medical Biller position 1. How to bill claims 2.
1. Identify the range of senses involved in communication • Sight (visual communication), Touch (tactile communication), Taste, Hearing (auditory communication), Smell (olfactory communication) 2. Identify the limited range of wavelengths and named parts of the electromagnetic spectrum detected by humans and compare this range with those of THREE other named vertebrates and TWO named invertebrates. Figure 1: the electromagnetic spectrum source: www.ces.fau.edu Vertebrates Human Japanese Dace Fish Rattlesnake Zebra Finch Part of electromagnetic spectrum detected ROYGBV (visible light) detected by light sensitive cells in the eye called rods and cones.
\section{Facility Static and Dynamic Control}\label{Calibr} The facility calibration is the transfer function between the oscillating gauge pressure $P_C(t)$ in the chamber (described in ~\autoref{Sub31}) and the liquid flow rate $q(t)$ in the distributing channel, i.e. the test section. Due to practical difficulties in measuring $q(t)$ within the thin channel, and being the flow laminar, this transfer function was derived analytically and validated numerically as reported in ~\autoref{Sub32} and ~\autoref{Sub33}. \subsection{Pressure Chamber Response}\label{Sub31} Fig.\ref{fig:2a} shows three example of pressure signals $P_C(t)$, measured in the pneumatic chamber.
On April 6, 2016 at approximately 11:45am, a local police station got a call about a hostage situation at a local pharmacy. When police and medical examiners got to each crime scene, they learned that all of the hostages were given drugs and had overdosed on them. Some of the pills, in powder form, were found near the victims. One of the victims was stable enough to tell the investigators that the power on the floor were the drugs they were forced to take. The medical examiner found out each hostage was given either unknown A or unknown B.
In this lab there were five different stations. For the first station we had to determine an unknown mass and the percent difference. To find the unknown mass we set up the equation Fleft*dleft = Fright*dright. We then substituted in the values (26.05 N * 41cm = 34cm * x N) and solved for Fright to get (320.5g). To determine the percent difference we used the formula Abs[((Value 1 - Value 2) / average of 1 & 2) * 100], substituted the values (Abs[((320.5 - 315.8) /
2.4 Band Division and Energy Computation: The power spectrum of the signal is multiplied by magnitude response of set of 33 triangular band pass filters and in the range 300Hz-2000Hz. Sub-bands are formed by using the logarithmic spacing. The positions of these filters are equally spaced along the Mel frequency, which is related to the common linear frequency f by following formula: Mel (f) = 1125* ln (1+f/700) (3) Mel frequency is proportional to the logarithm of linear frequency and which is close to the human perceptual system. 2.5 Sub Fingerprint Generation:
Tn 4351 was originally isolated from bacteroides fragilis [30] . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation [25]. Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides.
Typical sample dimensions 9.51 × 4.83 mm2in surface area and1.58 mm in thickness were coated with conductive silver paint formetallic contacts. The dielectric constant of the sample was mea-sured for the applied frequency that varies from 100 Hz to 1 MHz atdifferent temperatures (40◦C, 60◦C, 80◦C). The observations weremade while cooling the sample. The dielectric constant εrwas cal-culated using the relation, εr =
Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes.
Therapeutic drug monitoring (TDM) is the clinical practice of measuring specific drugs at timed intervals in order to maintain a relatively constant concentration in a patient's bloodstream, thereby optimizing individual dosage regimens. It is not necessary to use therapeutic drug monitoring for all the of medications, and it is used mainly for monitoring drugs with some narrow therapeutic ranges, drugs with marked variability in pharmacokinetic, medications with target concentrations which are difficult to monitor, and drugs that are known to cause therapeutic and adverse effects. The process of therapeutic drug monitoring is based on the assumption that there is a specific relationship between dose and plasma or blood drug concentration, and between concentration and therapeutic effects. Therapeutic drug
Practical I: Acid-base equilibrium & pH of solutions Aims/Objectives: 1. To determine the pH range where the indicator changes colour. 2. To identify the suitable indicators for different titrations. 3.
Decomposition of Aspirin Studied with UV/Visible Absorption Spectroscopy Aims: To determine the concentration of salicylic acid, formed from the hydrolysis of Aspirin, at regular intervals using the UV/Visible Absorption Spectroscopy From the concentration of salicylic acid, concentration of Aspirin to be determined using an equation Calculate the rate constant of this reaction and its order from a plot of graph of ln(aspirin) vs time Discuss the overall flaws and improvements to the experiment Results: As per schedule1, 0.212g of aspirin was added to 50 ml boiling water to form salicylic acid in a 100 ml flask, of which 1 ml was then pipetted to a 50 ml volumetric flask at the 5th min. Following an ice bath, the solution was mixed