Dosage Technique Lab Report

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CHAPTER 4

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH PDA DETECTOR FOR COMBINED DETERMINATION OF EMTRICITABINE, TENOFOVIR AND EFAVIRENZ

The present work describes a simple assay method for determination of emtricitabine, tenofovir and efavirenz in their combined tablet dosage form by using HPLC with PDA detector. Our aim was to develop a simple, accurate, sensitive method for simultaneous determination of emtricitabine, tenofovir and efavirenz in combined pharmaceutical dosage form by HPLC with UV detection, where simple mobile phase composition was used for chromatographic separation without any ion-pairing agent. Total retention time for analysis was short with a good resolution between emtricitabine, tenofovir and efavirenz. All these
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The output signal was recorded on the monitor and then quantified by using Empower2 Software. The separation was done by Agilent Eclipse XDB column (length 150mm × 4.6 inner diameter, 3.5µ particle Size) with a guard…show more content…
The quantity of tablet powder equivalent to 200 mg of emtricitabine, 300 mg of tenofovir and 600 mg of efavirenz was transferred to 100 ml volumetric flask consists of 30 ml of mobile phase. The solution was sonicated for 20 mins and made up to the mark with the mobile phase and then filtered through 0.45 µm membrane filter. The resultant solution was diluted appropriately with the mobile phase to get a final concentration of 200 μg/ml emtricitabine, 300 μg/ml tenofovir and 600μg/ml efavirenz.

Results and Discussion
HPLC parameters

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