INTRODUCTION
An ‘endophyte’ is often a bacteria or fungus that colonizes the internal tissue of the plant showing no external symptom of disease or harmful effect on the host (Holliday, 1989; Schulz & Boyle, 2006). In practical terms, an endophytic bacterium is that, which can be isolated from surface-sterilized plant tissue (Perotti, 1926; Henning & Villforth, 1940). The early literature on bacterial endophytes was dominated by studies on plants of agricultural importance such as rice (Sun et al., 2008), wheat (Conn and Franco, 2004), soybeans (Okubo et al., 2009), corn (Figueiredo et al., 2009), and potatoes (Garbeva et al., 2001). Significant differences have been found in the endophytic community of different crop cultivars (van Overbeek and van Elsas, 2008; Manter et al., 2010). These bacteria reside in the living tissues of the host plant in a variety of relationships ranging from symbiotic to pathogenic. Nature selects
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The story of this same species turning from being pathogenic to profitable specifically inside the plants laid foundation for our work. Considering Pseudomonas which is known to cause simple to serious infections in humans has identified to be beneficial for plants in many ways. The endophytic nature adapted by these bacteria, inside the plants can be understood only by studying the molecular basis responsible for compatible endophytic and plant interactions. Understanding the genome characteristics within the interactive stage would provide us clarity on this molecular basis behind the interactions. Culture-independent approaches are highly time conservating ways to study the genome characteristics. Rapid development n the sequence technology and higher throughput technologies provides quality data,
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
Throughout Michael Crichton’s novel, there are examples of how the government and research scientists involved were fearful of the foreignness of even the possibility of an extraterrestrial life form coming and ruining life for the people it effects. It is evident in both their research and experimentation and in their levels of protection for the outside world from the disease or lifeform being brought back to the facility. In Andromeda Strain, the microorganism called “Andromeda Strain” can be viewed as a metaphor for imperialism and signifies the fear of one culture being taken over by another. When Jeremy Stone discovers the structure of the microorganism, he notes how it is structured, but in a nature vastly different from any forms of
Purpose: To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods: Tests: Lactose fermentation: Fermentation makes energy available for use by microorganisms by anaerobic breakdown of carbohydrates. The product can either be an acid or gas. When it is positive, the broth will turn from red to yellow and if gas is present a bubble is formed.
A starch agar plate was inoculated with a streak of the unknown bacteria and then incubated. On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids. The smoke of the Iodine stained the plate to display the presence or absence of a halo around the bacteria 2.12 Lipid Hydrolysis This test was done by making a single line streak inoculation on a tributyrin agar plate and allowing incubation. After the incubation period, the plate was observed for the presence or absence of a halo around the bacteria.
The Unknown Identification Lab was an experiment that provided the opportunity to apply all the tests that were learned in the semester of lab, to identify the two bacterias that remain unknown. Gram- staining and two other tests will be used to identify the unknowns. This experiment is crucial to the understanding of each test, and can benefit in the ability to identify the characteristics of specific bacteria. Having a clearer understanding of the bacteria can further the research of bacteria for medicine, such as antibiotics. The understanding can also help the development of research in the environment.
Of the Enterobacteriaceae family, there are genera that are in the normal human flora. Some species such as K. pneumoniae and E. coli are opportunistic pathogens which can capitalize on weakened host defenses and cause food poisoning (Baron, 1996). S. enterica secrete proteins that help aid in intracellular invasion and proliferation (Hensel, 2009). K. pneumoniae is a part of the normal human mouth, skin, and intestine flora, but can wreak havoc if inhaled (Ryan,
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
Molecular analysis is a well-known method and recently used by researchers. Using this
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
By Gram staining alone, it was safe to eliminate the three Gram positive bacteria that could have been assigned: S. epidermidis, M. luteus, and B. megaterium. The second step was to streak plate Unknown #10 to observe its macroscopic
There are numerous ways from avoiding being infected by E. coli bacteria. The basis is to know how it is spread. Studies show that E. coli infections are food-borne. Having the organism live in the lower digestive tract makes the possible transmission between animals and humans. It happens when there is direct contact with animal infected by the bacteria or through consumption of contaminated products during food processing.
Escherichia Coli 0157: H7 This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge
Deep within the Amazon forests of Ecuador and Peru resides several elusive tribal groups that remain unaffected by the influences of globalization. More than 1 million ancestral people indigenous to the Amazon region can be divided into approximately 400 tribes that share their own specific language, territory, and culture. They heavily rely on the Rain Forest’s resources for the survival of their bodies and minds—but as modern day imperialism seeps its way throughout the globe and ravages the earth’s vast resources, the consequences and effects are beginning to reveal themselves. Throughout history, imperialism has been a major cause of conflict amongst various cultures, and it continues to shape the modern world. By understanding
The second ½ of the organism was used for gram staining. The gram stain method was performed on the unknown organism per lab manual page 42 and two gram stain reactions were identified. Organism B was gram positive cocci in grape like clusters. Because organism B was positive I could eliminate Escherichia coli, Enterobacter aerogenes and Proteus vulgaris because these bacteria would be rod shapes. Organism A was gram negative pink rod shaped and because of gram positive morphology I could eliminate Staphylococcus aureus, Streptococcus lactis and Bacillus subtilis.
Bacteria that causes disease are called pathogens. The disease is caused by a poison called exotoxin and endotoxin produced by the bacteria. Another microbial life are protist. They are unicellular eukaryotes. Types of protist includes protozoans and slime molds.