Three months later, the bone mineral density (BMD) of the femurs of all the rats were determined using GE Lunar Prodigy (GE Healthcare Bio Sciences, Pittsburgh, USA) to evaluate whether the model had been successfully established. Once the model is successful, the OVX rats were randomly divided into five groups of 14 animals: OVX group (double distilled water (0.2 mL/100 g, daily, administered orally)), positive group (70 mg/kg, weekly, administered orally) and OVX–HE groups (0.11, 0.33 and 0.99 g/kg, daily, administered orally) and treated for 12 weeks. The 0.11, 0.33 and 0.99 g/kg doses of HE were designated low (L-HE), medium (M-HE) and high (H-HE) groups,
Then, 3.7 µl of 10 mM Fluorescein Maleimide was added in equal quantities in wild type, mutant and purified ERAB protein, incubated at RT for 10 minutes. Similarly, 4.1 µl of 100 mM DDT was added in the three proteins respectively, incubated for 20 minutes again. And 3 µl of unmodified wt protein was transferred into a 1.5 ml tube as an additional sample. Then, 10.3 µl of 5 x SDS sample buffer was added to each sample and boiled for 5 minutes at 95 oC. Samples were cooled on ice for 1 min, spin down and now they were ready to load on the
To calculate the internal reliability of a scale Cronbachs alpha, α, is used. Cronbach’s alpha allows us to calculate the variance within an item and the covariance between any two items on the scale. For a scale to be deemed reliable α > 0.8. However, .7 is also acceptable. After testing the reliability, the α for all 12 items of the scale was revealed to be .82 which indicates good reliability.
While, 5µl of purified PCR product, 2µl buffer R, 1µl BamHI, 1µl HindIII and 11µl distilled water were added into SOD gene tube. Next, 1 µl of 0.2mg/ml RNase was put inside both tubes and spinned by using microcentrifuge for a few seconds. Those tubes were then placed into 37ºC water bath and incubated for 2 hours. The tubes were deactivated by heat at 65ºC for 10 minutes and stored in -20ºC. Results Measurement of DNA concentration Equipment Optical
Animals were also weight immediately prior to sacrifice (fasted body weight). Animals were sacrificed under anesthesia with diethyl ether, and then blood samples were immediately collected in clean and dried Wiesserman tubes from the portal vein. First part of blood was collected in tubes containing potassium oxalate and sodium fluoride for the estimation of plasma glucose by O-toluidine method of Sasaki et al. (1972). Second part of blood was left to coagulate then centrifuged at 3000 rpm for 15 minutes to obtain serum to estimate some biochemical parameters.
bronchiseptica biofilm formation was evaluated by using a microtiter dish assay following the methodology described previously . For each strain, 100 µl of a bacterial suspension adjusted to an optical density at 650 nm (OD650) of 0.05 were inoculated into the corresponding wells of microtiter plate. Plates were incubated statically at 36oC for 24 or 48 h. For 48 h cultures, the growth medium was entirely replaced with a fresh one after 24 h. To quantify biofilm formation, the liquid medium containing planktonic bacteria was firstly removed from each well. The remaining adhered biomass in every well was gently washed twice with PBS and subsequently stained for 20 min with a 0.1 % p/v Cristal Violet (CV) solution. After staining the CV solution was removed and every well was washed twice with distilled water.
“BEST GLYCOLYTIC INHIBITOR – D,L GLYCERALHYDE VS SODIUM FLUORIDE FOR ESTIMATION OF GLUCOSE” Author: DR.G.UDAYA KUMARI, PROF.V.MEERA, Department of Biochemistry, Kilpauk Medical College and hospital, Chennai. ABSTRACT: AIMS: To determine an ideal antiglycolytic agent - D,L Glyceraldehyde (GLY) or Sodium Fluoride(NaF) for estimation of glucose..MATERIALS AND METHODS: 80 random blood samples were collected from the OP patient after obtaining explicit consent. 6 ml of venous blood was extracted from each patient and was equally distributed between GLY tube, NaF tube and Plain tube with clot activator(PTC).GLY tube was prepared at the concentration of 5mmol/L.Serum from the PTC were separated after 30 minutes and refrigerated at 4°C until analysed.
Finally reviewing all the results it was concluded that concrete mixes with 6% micro silica and 8% metakaolin over 35% fly ash as base were the better mixes among all because compressive strength which is one of the key property of concrete obtained in these two mixes for M-30 grade concrete were close to or rather slightly higher than 30MPa. More over filling ability, passing ability and blocking ratio as obtained on other mixes were also well within the limits but were slightly on a higher side which may give rise to problem of segregation which is undesirable. Therefore studying all the test results we conclude the above two trials as the better amongst all. 7.2 SCOPE OF FUTURE WORK However, there are scopes which other scholars can work upon. The following points related to the scope of work with respect to the topics: In place of fly ash, micro silica and metakaolin to use rice husk ash, GGBS (Ground Granulated Blast Furnace slag), stone powder or any other materials.
Dengue infections can be asymptomatic or produce several syndromes that are conditioned by age and immunological status, the most common being an early febrile stage (including fever, malaise, headache, body pains, and rash), that can be followed by a tendency for bleeding that can progress to severe hemorrhages. Dengue is now considered a global health threat, endemic or epidemic in almost every country located in the tropics. It is estimated that there are over 100 million cases of dengue worldwide each year. Because dengue is a nationally notifiable disease, all suspected cases should be reported to the local country’s health
Discussion Dengue is now a global threat that has emerged as an important arboviral infection in different geographical regions of the world that supports the growth of mosquitoes. An estimated 50 million people live in dengue endemic countries. Its range exceeds over a hundred tropical and subtropical countries with more than 2.5 billion people at the risk of infection with about 100 million new cases each year worldwide.  [1, 19, 20] Dengue infection has been known to be endemic in India for over two centuries as a benign and self-limited disease. In north Indian region it has emerged as major health problem.