Environmental Factors Affecting Plant Tissue Culture

Environmental factors

Another factor that should be taken into consideration that affect the plant tissue culture is environmental factors. The control of the physical environment plays an important role in the success of tissue cultures. The variation in the pH, humidity, temperature, photoperiod as well as oxygen and carbon dioxide concentration also will give great effect on the growth of the plant tissue culture. These environmental factors are the factors that should be controlled during the process of culturing plant tissue. The physical state of the media plays an important role in the growth and the development of the plant tissue culture. There are different kind of media that has been used. Different kind of media depends on the

Some research found that a high humidity environment can lead to the disorders of the morphology and the physiology of the plantlets. As the humidity is high, the stomata cannot function efficiently in the process of evaporation. The plantlets might lose their ability to resist the water stress after the process of transplanting the plantlets from the in vitro to the ex vitro conditions in the high humidity environment. In one of the research done by the researchers, as they lowered the humidity, it can be seen that the stomata regulative had improved and the leaf resistance towards the dehydration also increased. A comparison of the plantlets growth in two different humidity level shown that the low humidity environment enhance the evaporation of plantlets compared to the high humidity environment. The humidity of the internal air in a culture vessel is also affected by the irradiance as well as the water content of the medium. The humidity also can be affected due to the presence of light. It can be seen that during light period, the air humidity is decreased and the tension of the medium increase while during dark period, the air humidity is a bit high causing the tension of the medium to increase causing more water to flow out from the medium and disturb the growth of the plantlets. The relationship between the relative air humidity and evaporation can be conclude that as the air humidity level is low, the rate of evaporation is high. In a conclusion, the relative air humidity will affect the physiology as well as the morphology of the