Enzyme Catalyzed Reaction Lab

Observing the effects of a catalyst on an enzyme’s rate of reaction

Leong, M., Kim, E., Nair, A.

Achilly, K., 9/22/2015

Introduction:

An enzyme is a protein that acts as a biological catalyst. A catalyst increases the rate of reaction by reducing the activation energy required (Reece 2005). Catalase, an enzyme produced by most living organisms, catalyzes the decomposition of H2O2 in our bodies in order to maintain homeostasis. Enzyme activity involves the binding of an enzyme to a substrate at its active site. Each active site is different and unique to its substrate, which is often thought similar to a lock and key. An active site can deform and denature through extreme levels of conditions such as salt concentration, pH, and temperature, becoming biologically inactive (Article 19-21). Another key concept is titration, which is the

The trials of timed intervals showed that the independent variable, time, is directly proportional to the dependent variable, amount of H2O2 used. Compared to the uncatalyzed reaction of H2O2, the enzyme-catalyzed reaction had a much faster rate of decomposition.

ADD A RESOURCE/SECOND SOURCE TO COMARE TO

State whether hypothesis was supported or not (DON’T RESTATE HYP). Explain the effects of the dependent variable on the independent variable Relate results to overall experience- how it furthered your knowledge in enzymes and catalyzed rxns. DON’T REPEAT RESULTS

Literature Cited:

Cooper, Geoffrey M. "The Central Role of Enzymes as Biological Catalysts." The Central Role of Enzymes as Biological Catalysts. U.S. National Library of Medicine, 2000. Web. 21 Sept. 2015.

"Two Enzyme Catalysis." Article. n.d.: 19-21.

Reece, Jane B., Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, and Robert B. Jackson. "Concept 8.4." Campbell Biology AP*. 9th ed., 2005. N.p.: Pearson, n.d.