Enzyme Concentration Lab Report

For example, an experiment similar to this one could be how pressure could affect the time it takes for an Alka-Seltzer tablet to dissolve. Using A flask and rubber stoppers, you can compare the amount of time it takes for and Alka-Seltzer tablet to dissolve in regular room temperature water and room temperature water in a flak but with a rubber stopper stopping the air flow into the flask. This is related to the experiment performed above because the increase in pressure from the rubber stopper stopping the air flow in and out of the flask may affect the time it would take for the Alka-Seltzer tablet to dissolve. Another example of an experiment related to the one performed above is how the amount of water used to dissolve an Alka-Seltzer tablet effects the time it takes for one to dissolve. This also relates to the experiment performed above because it also affects how fast the rate of reaction is.

The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.

H20 + 2 O2 This experiment will use 1% catalase solution and 3% hydrogen peroxide solution, both diluted into water so the reaction slows down. Temperature will be controlled in this experiment to change the reaction speed of the enzyme and the substrate, this is what the experiment is looking at. The effect of the temperature will be determined by how much gas is released in two minutes, which will change the pressure inside the test tube and will be measured by a gas

Enzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. Enzymes are fragile and easily disrupted by heat or other mild treatment. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside (ONPG) used in place of lactose. Upon hydrolysis by B-galactosidase, a yellow colored compound o-Nitrophenol (ONP) is formed.

The enzyme found in different conditions which its specific reaction rate. Variation in enzyme concentration, variation in pH, variation in temperature, and the effect of different concentrations of inhibitors were all tested. The enzyme concentration increased the reaction rate. An optimum pH and temperature were found for the enzyme, outside of this optimum the reaction rate would be lower. The correct name is the catalase enzyme.

Malate dehydrogenase: Malate dehydrogenase (MDH) is an enzyme in the citric acid cycle that catalyzes the conversion of malate into oxaloacetate by using NAD+ and vice versa and this is a reversible reaction. Malate dehydrogenase is not to be confused with malic enzyme, both are different enzymes malic enzyme which catalyzes the conversion of malate to pyruvate and producing NADPH. Malate dehydrogenase is also involved in gluconeogenesis, in which the synthesis of glucose from smaller molecules. Pyruvate in the mitochondria is based upon pyruvate carboxylase to form oxaloacetate, a citric acid cycle intermediate. The malate dehydrogenase reduces it to malate, and it then traverses the inner mitochondrial membrane to get the oxaloacetate out

Lastly, the other fallacy derives from the water temperature. As the temperature of the water increases, carbon dioxide is produced up until its optimal temperature. In reality, when the plant attains the optimum, denaturalization of enzymes in elodea occurs so that that leads to cessation of oxygen. Similar to this situation, same result would be created when the temperature is too cold.

Concentration of acid and reaction time Aim In this experiment, I’m going to find out the relationship between concentration (mole) of acid and carbonate’s reaction. Hypothesis If the concentration of acid is stronger, then the film canister will pop (react) faster. Because the concentration of Sodium carbonate is always same in my experiment, so the factor that changes the volume of carbon dioxide is concentration of Hydrochloric acid.

acetyl CoA + ATP + HCO-3 □(⇔┴( BIOTIN ) ) Malonyl CoA +ADP+ Pi This is designated as Bi, Bi , Uni, Uni, ping pong mechanism because first two substrates add to the enzyme, then two products are released, then another substrate adds and the final product is released. Acetly coa carboxylase catalyzes coupled reaction.

On a glass slide, 1 drop of distilled water was placed. Then, a loop-full of culture was transferred on the slide and it must be spread. It is then allowed to dry. Then, the smear must be heat fixed by exposing it to flame for few times until it got fixed. It is to prevent the cell from washing away during the staining and washing process.