Enzyme Concentration Lab Report

Lab Report -- Relationship on Enzyme activity and substrate concentration

Research Question:

Is the more concentrated the substrate of hydrogen peroxide is, the shorter the time taken for the paper disc to rise from the bottom of the beaker?

Aim:

The opposite of hull hypothesis

Background Information:

This experiment aimed to investigate on the relationship of the substrate concentration and enzyme activity. Enzymes are proteins produced by a cell that acts as catalysts to increase the rate of a specific chemical reaction without changing the reaction itself. Under some conditions, substrate will bind to the active site of an enzyme and form an enzyme-substrate complex. The enzyme would fasten the chemical reaction and the substrate will

The dry yeast is dissolved in water become a mixture and filter paper discs are dip into this catalase solution. Then, the discs would contain catalase and be put into the bottom of the hydrogen peroxide where the chemical reaction would take place. Because of catalase, the reaction would take place quickly and oxygen, one of the products of the reaction, would stay on the surface of the filter paper disc making its density to decrease. When the oxygen produced has reached to a certain level where the density of the paper disc is lower than water, it would allow the filter paper discs to float and rise to the surface of the hydrogen peroxide. As a result, through measuring time taken for the paper discs to rise to the surface in different substrate concentrations means measuring the rate of oxygen production as the volume of oxygen needed for lowering the density should always be the same, hence, the effects of substrate concentrations on the rate of enzyme reaction can be

If it is affected, the experiment is not fair anymore.

Size of beaker mL Using same volume of beakers

To ensure that the diameter of the beakers are the same so that when same amount of hydrogen peroxide is poured into the beaker, the height of the solution would remain the same. As if the height changes, the distance the discs has to rise would change which would affect the time taken to reach to the surface.

Volume of water mL Use a measuring cylinder to measure

To make sure that the amount of catalase on the filter paper disc are nearly the same every time as the volume of water would affect the production of catalase.or The time taken for the disc to rise to the surface would change if it is different every time.

Volume of yeast mL Use an electric balance to measure

To make sure that the amount of catalase on the filter paper disc are nearly the same every time as the volume of yeast would affect the production of catalase. This would affect the time taken for the disc to rise to the