INTRODUCTION Enzymes are biological catalyst that alters the chemical reaction rate without itself being altered which reacts with the substrate and converts the enzyme substrate complex into different molecules – product. Enzyme plays the consequential role in functioning of life process such as for growth, digestion of nutrients, excretion of metabolic waste, energy provider to brain and muscles and thus directly or indirectly involved in every biological processing of life. Apart from numerous life functioning role, enzymes are also used in industry-oriented procedure such as for drug delivery in biomedical research, production of biodiesel in energy sector, production of jams and syrups in food industry, treatment of sewage in waste management
The three things that can cause the enzyme to denature is a large change in pH level, High Temperature, and substrate concentration. According to our knowledge, we know that a large change in pH will cause instability in the protein structure thus resulting in denaturation of the enzyme. From the data, we can see that pH 3 (total:6.3) and 10 (total:6.2) were the slowest because pH 3 is probably the highest acid and pH 10 is the highest base. The highest acid or base pH represents a large change which would cause the enzyme to denature. The fastest pH was 6 (total:34.5), and it seems that there wasn’t a large change which resulted in a stable structure.
Enzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. Enzymes are fragile and easily disrupted by heat or other mild treatment. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside (ONPG) used in place of lactose. Upon hydrolysis by B-galactosidase, a yellow colored compound o-Nitrophenol (ONP) is formed.
Introduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).
Starting off, the greatest upside of being a chemical engineer is that they have the highest paycheck of any type of engineer. Aside from the ups and downs of a job, people looking for a career should consider what things they would do on a typical day. In this case, they would find that chemical engineers design, construct, and operate equipment to produce products on a large scale. Also, they estimate costs, effects of processes on the environment, and staff needed for a procedure. Along with estimations, they calculate temperature and pressure throughout a process.
Enzymes are defined as biological catalysts since they are protein molecules that are produced by living cells. A catalyst is a substance which speeds up a chemical reaction but remains unchanged itself at the end. Enzymes are biological catalysts because they increase the rate of chemical reactions in cells which would otherwise be too slow to sustain life. Several factors affect the rate of enzyme reactions. These include: temperature, pH, enzyme concentration and substrate concentration.
Catalyse Enzyme Experiment. Enzymes are biological catalysts. They speed up chemical reactions which go on inside living things. Without them reactions would be so slow that life would grind to halt. These are examples that can decomposition of the hydrogen peroxide.
Substrates are molecules that enzymes could act upon and the enzyme converts the substrates into different molecules known as products. Enzyme catalysis is needed in almost all metabolic processes in order to happen in rates/ways that are fast enough to sustain life. (wikipedia. 2018. enzyme. [ONLINE] Available at: https://en.wikipedia.org/wiki/Enzyme.
Enzymes are biological molecules, proteins, which act as catalysts and help complicated reactions occur everywhere in life. Enzymes are very precise catalysts that usually work to complete one assignment. Example being; an enzyme that helps digest proteins will not be useful to break down carbohydrates. Also, you will not find all enzymes everywhere in the body. They are also precise on the temperature they work best in which is usually about 35 to 40 degrees.