Some research has indicated that a lack of catalase can lead to the development of type 2 diabetes. It seems that some other molecules within living organisms are able to sufficiently break down hydrogen peroxide—enough to sustain life. The toxic nature of hydrogen peroxide also makes it a powerful disinfectant. And in conclusion from the information ive found ,catalase functions best at around 37 degrees Celsius as the temperature gets colder or hotter than that, the ability to work will denature and the enzyme will be
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate. All enzymes are under the class of protein biomolecule. Amino acids are the basic units that are combined to make up an enzyme.
Introduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).
To represent the blood of a person with ketoacidosis, we used a liquid that had a high pH level. Using the Lugol’s Iodine test, we investigated the effect of an environment with a high pH on the function of enzymes. In our experiment we added 15 drops of starch (amylose), 15 drops of an enzyme that breaks up starch (amylase), and 15 drops of the high pH liquid into a test tube. Next, we added 15 drops of the Lugol’s Iodine solution into the same test tube. We measured how long it took for the high pH test tube to change color versus how long our control took.
Introduction: What are enzymes? Chemical reactions that take place in living cells are known as metabolic reactions. There are two types of reactions: • Anabolic Reaction (Constructive) • Catabolic Reaction (Destructive) Substance that accelerate chemical reactions are known as catalysts. Enzymes are biological catalysts, because they accelerate chemical reactions that occur in cells. Activation energy is required to start a chemical reaction.
Metabolism is the chemical process that occurs in every living organism to maintain its life. Without it, an organism would ultimately die because metabolism is the powerhouse of an organisms body, creating energy to make it work and function. There are two different processes that occur in metabolism, these processes are called catabolism and anabolism. In the catabolism process molecules breakdown, these molecules include carbohydrates, proteins, and fats. When they breakdown they go into a simpler form, which is then used to create energy that the body can use.
A 60°C water bath was chosen as Sodium Hydrosulphite will decompose into Sodium Sulfate and Sulfur Dioxide in presence of air at 90°C. After soaking a piece of cotton for an hour, it was taken out to be oxidised through exposure to air. Oxidation converts Leucoindigo back into Indigo, and a colour change from yellow to green to purple was observed. This is because Leucoindigo absorbs light poorly in the yellow(570nm) wavelength, while Indigo absorbs light in the blue(475nm) wavelength. The visual change in colours can be seen due to the changes in amount of each form as oxidation
As a candle burns out much faster in oxygen than in air, the body becomes quickly exhausted in this “pure kind of air.” The toxicity of the atmospheric oxygen molecule had already been used by our ancestors for therapeutic purposes, such as treatment of places infected with the anaerobic bacterium Clostridium by exposure to air (Halliwell B, Gutteridge JM, 1999). The valuable use of the toxic effect of oxygen has been applied in hyperbaric and irradiation therapy (von Sonntag C, 1987). The advancement of submarines, diving as a sport, and medicine has contributed much to the knowledge of oxygen, its derivatives, and its toxicity (Balentine JD, 1982). Cases of blindness in young infants born prematurely in the 1940s were associated with the high oxygen concentration in the newly invented incubators (Gitto E, Reiter RJ, Karbownik M, Xian-Tan D, Barbereri I, 2001). This pathology, called retinopathy of prematurity (ROP), was easily controlled by balancing the oxygen concentration in the incubators.
Abstract: Drug companies must apply the knowledge gathered from the effects of substrate concentration in an enzyme catalyzed reaction. The awareness of inhibitors must be applied so that their developed drugs do not inhibit enzymes. It would be important to consider substrate concentrations in relationship to target enzymes that are exposed. Competitive inhibitor drugs compete with high concentrations of ATP in the cell and proteins inside the cell contain lower concentrations. Thus the knowledge of the effects of substrate concentration on enzyme activity would aid drug designers in utilizing competitive inhibitors that will inhibit the enzyme more effectively.
The rate actually depends on the concentration of hydrogen peroxide raised to a power, called the "reaction order." Equation 5: Rate = k(H2O2)x • k = Rate constant, in 1/seconds (s) • (H2O2) = Concentration of hydrogen peroxide, in moles/liter • x = Order of the reaction for hydrogen peroxide, unit less The good news from Equation 5 is that the rate depends on the concentration of hydrogen peroxide, and you will know what the concentration of hydrogen peroxide is when the reaction starts. You will use the number of hydrogen peroxide drops as a measure of its concentration. *insert aim* Hence I’ve arrived at the following question: How does varying the concentration of hydrogen peroxide affect the rate of reaction? Research Question: How does varying the concentration of hydrogen peroxide affect the rate of reaction?
6. How would you design an experiment to show how much faster H2O2 decomposes in the presence of an enzyme then it does without the enzyme? Use the same system and just add it with water and compare both of them. 7. Explain why the enzyme is still active even though the liver cells from which you obtained the enzyme were no longer living?
If I had a chance to do this experiment once again. I would improve this experiment by actually testing, whether temperature affects the rate of reaction of the alka seltzer tablet, by having the water be hot, warm and cold, and determine at which temperate does the tablet dissolve faster. I could also change the amount of water I used during the experiment, as I only used 100ml of water in a 400ml beaker. I would also change the temperate of the room. A further investigation can study how temperature affects the rate of reaction between water and alka seltzer tablets.
The constants of the experiment, will be the amount of water used and the Alka Selter compound. The control in the experiment is water. Units used while timing the productivity of gas from an Alka-Seltzer tablet in different temperatures is, seconds. In order to find out if temperature controls the rate of chemical reaction, whether hot water is a more effective way to make the gas produce at a faster speed, it would be necessary to compare the results of different temperatures at the end of each trial. In order to do this the scientists will measure the volume of gas that is produced within a 10 second interval time after the tablet begins to react.
An experiment was conducted in order to determine the oxygen consumption in dry and soaked peas within two different temperatures of water. Cellular respiration is the release of energy by metabolic chemical oxidation in the mitochondria, (DeStefano 2016). The hypothesis was the germinating warm water peas would have the highest rate of oxygen consumption. Within this experiment, a twenty-one degree celsius bath and a six degree celsius bath were set up. Then, a one hundred mL graduated cylinder was filled with fifty mL of water.