Substrate concentration basically means the amount used for the substrate. The substrate in our experiment was 0.1% hydrogen peroxide. The 0.1% is the concentration amount. Just like temperature and pH, substrate concentration can speed the reaction only up to a certain limit. When we mixed pH 3 enzyme tube with substrate tube, we used 0.3 mL of hydrogen peroxide, but if we were to increase the amount, then the experiment would have been faster.
With that being said, since potassium is a mineral and nearly 98% of the ions are found within the cells, even the slightest bit of changes in the level of potassium that is found outside the cells may also cause severe effects on the heart, nerves, and muscles. The control of potassium is regulated by removing the excess amount of it into the
Controlled Variables temperature, pH, sucrase + sucrose incubation time 4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. I believe glucose and fructose was used as an indicator because they are what produces sucrose and sucrose creates more sucrase activity. 5. Explain why denatured sucrase was used as a control.
(Slideshare.net, 2016) The peak is associated with the onset temperature, which the substances starts to melt. The area under the peak is the enthalpy of the phase transition. So the enthalpy or the changes is equal to the heat supplied by the system (Figura and Teixeira, 2007). In conclusion, melting is the latent heat of fusion and also the phase transition of the substances from solid state to liquid state. Melting point is very important in foods because it affect directly the quality, taste and also appearance of
As a result the presence of the enzyme catalase in the cell helps to quickly convert this toxic substrate into safer products of water and oxygen (All Science Fair Projects, 2004). Hypothesis: According to the Collision Theory, in order for a reaction to occur, particles need to collide with a sufficient amount of energy and with the correct orientation. The temperature of the particles can affect the rate at which the particles collide. As you increase the temperature, the rate of reaction increases. This occurs because as you heat a substance, the particles move faster and
Hypothesis: Increasing substrate concentration will increase the initial reaction rate until it stops increasing and flattens out. Independent Variable: Substrate concentration Dependent Variable: The substrate itself, 1.0% Hydrogen Peroxide How Dependent Variable will be Measured: Hydrogen Peroxide will be used in every experiment, just with different test tubes. The amount of Hydrogen Peroxide in the mixing table is the amount that will be added to each test tube. Rationale: Enzymes are used to make reactions faster. When there is more substrate the enzyme will be forced to work harder to get rid of it faster.
If the state of ionization of amino acids in a protein is altered then the ionic bonds that help to determine the 3-D shape of the protein can be altered. This can lead to altered protein recognition or an enzyme might become inactive and denatured. Changes in pH may not only affect the shape of an enzyme but it may also change the shape or charge properties of the substrate so that either the substrate cannot bind to the active site or it cannot undergo catalysis. Several factors are influenced directly by the pH in which the reaction takes place. Extremely high or low pH values generally result in complete loss of activity for most enzymes.
Any Increase in substrate concentration after this point cause further increase in the rate of reaction because at Vmax enzyme moleclues are completed saturated with substrate molecules. 3.Effect of temperature At very low temperature enzymes are inactive.Enzymes activity increase gradually with the rse in temperature until a temperature at which the enzyme attains its maximal activity this temperature is called optimum temperature which lies between 37-40 c in humans. 4.Effect of pH Each enzyme has an optimum pH which it attains its maximal activity
As much was conducted throughout this lab, the projected completion of this lab displays that ultimately, the higher the temperature of the water, the faster the dissolving rate of the Alka-Seltzer is. In other words, the hotter the water temperature the quicker the tablet dissolves within the water in regards to the amount of time it took to dissolve. Furthermore, this experiment helps to explain that, if water is taken at a higher temperature and Alka-Seltzer is placed within the water, the Alka-Seltzer will take less time to dissolve because the higher temperatures cause the tablet to melt at a quicker rate. This compares to when Alka-Seltzer is placed in colder temperatures, where instead it takes more time to dissolve, because the lower
In the plasma acid degumming process, the pectin degraded. When processing temperature increased from 20 oC to 90 oC, the effect of plasma acid was enhanced. According to the pectin removal rate, processing temperature and time were the important factors. Under 1:10, 90 oC, and 80 min (A1 B3 C3) condition, the pectin removal rate was better. In plasma acid processing, lignin swelled and further dissolved, including aromatic ring fracture and the molecular weight decrease.