The cup was then covered and the temperature probe inserted. While the probe was collecting a few initial temperature readings for the solution, 50mL 2M NH4Cl was prepared but not added to the solution. After 3-4 readings were collected, the 2M NH4Cl was added to the solution. The lid was quickly replaced, preventing heat from escaping and not being recorded by the temperature probe. The cup was swirled until the temperature reached a peak and
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate. All enzymes are under the class of protein biomolecule. Amino acids are the basic units that are combined to make up an enzyme.
Lab Report -- Relationship on Enzyme activity and substrate concentration Research Question: Is the more concentrated the substrate of hydrogen peroxide is, the shorter the time taken for the paper disc to rise from the bottom of the beaker? Aim: The opposite of hull hypothesis Background Information: This experiment aimed to investigate on the relationship of the substrate concentration and enzyme activity. Enzymes are proteins produced by a cell that acts as catalysts to increase the rate of a specific chemical reaction without changing the reaction itself. Under some conditions, substrate will bind to the active site of an enzyme and form an enzyme-substrate complex. The enzyme would fasten the chemical reaction and the substrate will
We then slowly added 25ml of chilled deionised water to the filtrate to initiate crystallization by using a measuring cylinder and a dropping pipette, once we had done this we left it for about 10 minutes to allow crystallization at room temperature. We then weighed a filter paper which we will use later in the experiment. We then collected the crystallized acetylsalicylic acid by vacuum filtration in a Buchner funnel and washed the product with a little ice-cold water. We then pre-weighed a clean, empty watch glass and labelled it with our initials and the date, we did this do we could easily identify that it was ours when we go to weigh it with the crystals on. We
Hydrogen gas was generated during the reaction which was seen when bubbles were formed as the penny was dissolved into the beaker. An error that could have been present during the lab includes not letting the zinc react completely with the chloride ions by removing the penny too early from the solution. For instance, the percent error of this lab was 45.6%, which was determined by the subtraction of the theoretical percent of Cu 2.5% and the experimental percent of Cu 3.64% and dividing by the theoretical percent of Cu 2.5%. This experiment showed how reactants react with one another in a solution to drive a chemical reaction and the products that result from the
When carbon dioxide reacts with water, carbonic acid is made. This 0.1% aqueous bromothymol blue solution (also known as Bromthymol Blue) is a commonly used pH indicator. Bromthymol blue changes color over a pH range from 6.0 (yellow) to 7.6 (blue). It is a good indicator of dissolved carbon dioxide (CO2) and other weakly acidic solutions. Despite its name, bromothymol blue solution may sometimes appear yellow or reddish depending on the pH of the stock water used to prepare this pH indicator solution.Low levels of carbon dioxide or acid in solution with bromothymol blue indicator will appear blue.
Introduction The goal of the experiment is to examine how the rate of reaction between Hydrochloric acid and Sodium thiosulphate is affected by altering the concentrations. The concentration of Sodium thiosulfate will be altered by adding deionised water and decreasing the amount of Sodium thiosulphate. Once the Sodium thiosulphate has been tested several times. The effect of concentration on the rate of reaction can be examined in this experiment. The chemical equation for this experiment is hydrochloric acid + sodium thiosulphate + deionised water (ranging from 25ml to 0ml in 5ml intervals) sodium chloride + deionised water (ranging from 25ml to 0ml in 5ml intervals) + sulphur dioxide + sulphur.
Lastly, it told us to repeat the same steps until we had three calcium chloride scoops in the beaker and repeat for two more trials for accurate results. To sum up the experiment, it said to record the average change in temperatures to the class averages to graph a bar graph comparing both of the averages. That’s the procedure on how to conduct the experiment correctly. The averages that my group received for zero scoops were 0.5 degrees Celsius, one scoop was 6.5 degrees
The topic that the scientist has researched is the reaction rate of different particle sizes. In the experiment, the scientist will discover how the particle size of Alka Seltzer affects the rate of chemical reaction with water. The independent variable in the experiment is the particle size of the Alka Seltzer, while the dependent variable is the rate of reaction, or the volume of Carbon dioxide. The volume of carbon dioxide will be measured in ml. Also, a few of the constants in the experiment will be the amount of water, and amount of tablets.
An enzyme is a biological catalyst (protein) which speeds up the rate of chemical reactions without changing the chemical reaction at the end. A chemical reaction is when a substance is changed into a different substance. To begin a reaction, you need energy which in this case is called activation energy. A reaction in a chemical reaction is called a substrate when it is being acted upon by an enzyme that speeds up the rate of a reaction. In addition, the region on the enzyme where the substrate binds is the active site.
Four randomly selected Daphnia magna, for each trial, were removed from the provided colony for the bioactive compounds to be tested, and were transferred with a plastic wide-mouth pipette with approximately 10 mL of pond water to protect and ensure survival of the Daphnia. In order to acclimatize the Daphnia to laboratory conditions, they were then placed onto a petri dish on the Daphnia cooling chamber. The cooling chamber was located on the stereomicroscope platform and brought down the heart rate of the Daphnia to a range that was countable by the observer, since Daphnia heart rate at room temperature is too rapid. On the cooling chamber there were two petri dishes: one for the Daphnia that were going to be tested, and one with the Daphnia being tested on, to ensure constant consistent temperatures for each trial. To maintain a temperature conducive to the heart
Set the wavelength to 470 nm, this is to measure the tetraguaiacol. Set the spectrophotometer to zero by using a blank. The blank should contain 13.3 mL of distilled water, 0.2 mL of guaiacol, and 1.5 mL of enzyme extract in a clean test tube. After, transfer a portion of this mixture into a cuvette, cover the top of the cuvette with Parafilm and then place the cuvette into the spectrophotometer and set it to
After each 1 mL the solution is tested for acidity with red litmus paper. When the litmus paper final appear blue, the stirring is stopped and no more 6 M NaOH is added into the beaker. After each addition of 6 M NaOH the solution in the beaker becomes a thicker and darker