Enzyme Rate Of Reaction Lab Report

Experimenting the Effects of pH Levels on a Liver’s Enzyme Rate of Reaction

What is the effect of pH levels with variations of 1.0, 2.0, 5.0, 7.0, 9.0 (±0.5) on the effect of rate reaction measured by volumetric amount in mL (±2.5) when time (180 seconds), temperature (23ºC) and substrate concentration (1g of 100%liver (±0.01)) are kept constant? This experiment was successfully conducted by placing the pieces on liver inside several flasks holding different pH levels and measuring the amount of oxygen released by the reaction by collecting it in a balloon and later calculating its volume.

Criterion 1: Personal Engagement Human anatomy has always been a fascinating topic for me. My interest began when my left kidney started

Both Hydrogen Peroxide and Sodium Hydroxide are materials that irritate the skin when in

Criterion 3: Analysis:

Table 1 (Raw Data): Volumetric amount of oxygen released due to reaction through 5 different pH levels, each having trials Volumetric amount of oxygen (mL ± 2.5) Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 pH 1 60 69 55 35 45 pH 2 106 92 80 82 97 pH 5 310 320 315 296 322 pH 7 885 874 875 905 860 pH 9 1176 1210 1200 1185 1240 Controls and Qualitative data:

Table 2 (Processed data): Volumetric amount of oxygen released due to reaction through 5 different pH levels, each having 5 trials with average results and standard deviation.

Volumetric amount of oxygen (mL ±

I concluded that as pH increases, the enzyme’s rate of reaction increases as well. For example, this is illustrated in the data by the fact that the liver at pH 9 released over 1000 more mL of oxygen than the liver at pH level 1. This also aligns with my expected results, because I predicted that pH would cause rate of reaction to increase. The rate of reaction increases along a curve, suggesting rate of reaction increases faster than pH increases. Also, the data shows that there is a very large difference between the amounts of oxygen released by the varying pHs, even though the difference in pH is not so great. For example, between pH 5 and 7, there is an almost 500 mL difference, even though they are only 2.0 away on the pH scale. This shows even small changes in pH can have a large difference in the rate of reaction.

Evaluation of Conclusion: The data and conclusion does match with previous background research. Background research suggests that reaction rate increases with pH until a point where optimum pH is reached, after which the enzyme is denatured and no longer can perform its function. This research was also shown in the data, even though the point at which the enzyme denatured was not