Enzyme Reaction Lab

Discussion/Conclusion:

The question of the experiment was the effects of concentration, temperature, pH lever, and inhibitors of enzymes that increase the rate of a chemical reaction without making a change itself in living cells. In the experiments, peroxide was used as the enzyme. The hypotheses were:

1. Concentration of the extract would directly affect enzyme activity.

2. Rise in temperature would rise the rate of enzyme activity and absorbance until the temperature hits the optimal temperature,

3. pH level would affect enzyme activity,

4. and, the existence of inhibitor would decrease rates of the enzyme activity.

According to the figure 1, higher concentration of the enzyme extract brought higher absorbance. The slopes of the graph show

Enzyme with the least extract, 0.5mL, had the lowest absorption rate at the end. However, it had a higher rate than 1 ml ones until 80 seconds passed. I assume that decrease of the absorbance rate as it is fully saturated and cannot absorb more like other two controlled enzymes with larger amounts of extracts. According to the figure 2, reaction rates and concentration of enzyme extracts are directly related. As the amount of extract increases, the reaction rate also increases constantly. In conclusion, the test and figures one and two supported that the hypothesis 1 was correct: The concentration of enzyme extracts directly affects enzyme activity. According to the figure 3, absorbance keep increases no matter the temperature, but slopes are varied by the control. The coldest enzyme at 4 C had the highest absorbance, and the slope is also steeper than others. Then the observance decreases with the increase of the temperature, until 42 C. 4C is having highest, then 25C, 32C, 60C, and 42C had the lowest absorbance. The graph shows that as temperature increases absorbance decreases until 42C. Temperature affects the

According to the figure 5, pH 5 had the highest absorbance and pH 3 had the least. pH 7 had the second highest absorption after 2 minutes of measurement. According to the graph, I assume that, absorption would decrease as pH reaches two ends of the scale. pH 3 and pH9 were two lowest pH tested in enzymes and the had lowest absorbance at the end. Enzymes with a pH level around the middle of the scale (pH6~8) had steeper graph lines than others, which means as pH level reaches to the neutral, the absorbance increases. The level of pH affects absorption of the peroxidase. According to the figure 6, closeness of pH 5 to 7 determines the enzyme’s reaction rates. It had the lowest reaction rate is at pH 3 and 9 while it had the highest at pH 5. In conclusion, the enzyme reaction rate is higher at the neutral state of pH and the pH level affects the enzyme’s activity. Therefore, I accept the hypothesis 3 which was the pH level would affect enzyme