[Figure 1] 2.2.1. Chloromethylation of poly sulphone 5 g of polysulphone was dissolved in 75 mL of chloroform at 70°C. After complete dissolution of polymer, a mixture of paraformaldehyde (3.4 g) and chlorotrimethylsilane (14.7) mL was prepared as the chloromethylating agent with constant stirring which was followed by the addition of 5% of stannous chloride (by weight, of polymer) as catalyst with stirring at 70°C and allowed to react for 18 h. Then the polymer was precipitated in methanol to eliminate
You must first test the pH level of the amylase and starch solution using pH test strips, so that the experiment may be fair m. Then measure 3cm3 of amylase solution using the measuring cylinder, the pour it into the test tubes labeled A1-A5 n. Do the same for the starch solution but pour into the test tubes labeled S1-S5 o. Put test tubes A1 and S1 into the beaker labeled “cold water” p. Put test tubes A2 and S2 into the beaker labeled “normal water” q. Put test tubes A3 and S3 into the beaker labeled “warm water” r. Put test tubes A4 and S4 into the beaker labeled “very warm water” s. Put test tubes A5 and S5 into the beaker labeled “hot water” t. Mix the amylase solution with the starch solution when both are at the same temperature in each beakers (pour the amylase solution into the starch solution) u. Quickly add 3 drops of iodine solution into all 5 mixed amylase and starch solutions, while starting the stopwatch for each (should be 5 separate
13) Set the spectrometer to a wavelength of 530 nanometers. 14) Place the cuvettes (numbers 1-6) with the appropriate substance and record it’s reading in the data table. 15) After each cuvette was tested, place the distilled water sample (Cuvette zero) to reset the spectrometer and to ensure that the scale is calibrated and repeat for each cuvette test. Data/Results: Tube Number Concentration Of CoCL2 (Mg/ML) CoCL2 Stock (ML) Distilled Water (ML) Spectrometry Reading at
This would allow you to observe the drops of the distillate that enter to Graduated Cylinder. Step 2: Begin distillation. ➢ Select the flask, and then choose 50 mL of crude oil from the Chemicals menu. Then, by selecting the flask and choose “Chemical Properties” option from dropdown. NOTE: Record the grams of gasoline, kerosene, and lubricating oils that are present in the 50 mL of crude oil.
Each of the analyte will have its own Rf value under certain circumstances. The separation of the phospholipid classes can be improved by two-dimensional chromatography. This technique requires developing the TLC plate in a direction, then dried, and developed in a solvent mixture at a 90 ° the first development (Singh and Jiang,
The setup for the cation exchange chromatography is shown in Figure 3. This was done by plugging the bottom of a burette with a small amount of glass wool. The wool was lightly packed using a thermometer. Approximately 5 mL of Dowex 50 cation exchange resin was obtained in a small beaker, and the resin was mixed with 5 mL of pH 3 citrate buffer. This mixture was poured into the burette with the stopcock closed.
¾). Placed the cuvette sample in the Sprectrovis. After each run, the temperature of each sample was collected (to nearest 0.1°C). Disposed of the sample solution, cleaned the cuvette with DIW and repeated the latter procedure using the correct volumes for each new run from Table 1.
3. To identify the unknown acid. 4. To determine acid dissociation constant, Ka and pKa for the unknown acid. Introduction: Titration process is used in an acid-base experiment in order to determine the concentrations of solutions of acids and bases.
Remove the plunger from the syringe barrel and place the ten leaf discs into the syringe barrel. 10. Place the plunger back into the syringe barrel then carefully push the plunger down until there are 3cm3 of air are left inside (make you sure no leaves are crushed) 11. Pull 5cm3 of Sodium hydrogen carbonate into the syringe. In the case of the leaf discs being stuck on the sides of the barrel, tap the syringe to suspend them in the solution.
7- Once finished turn the Bunsen burner to a safety flame and turn off the gas Materials- precipitates test In this experiment you will need: -Test tubes -Test rack -Unknown solution A -Unknown solution B -Silver Nitrate -Safety goggles -Barium chloride Method- precipitates test The method to this experiment is: 1- Gather your materials 2- Put on your safety goggles 3- Get unknown solution A and pour centimeter into a test tube 4- Add a centimeter of silver nitrate into unknown solution A 5- Observe the reaction and record your notes 6- Pour a centimeter of unknown solution B into another test tube 7- Add a centimeter of silver nitrate into unknown solution B 8- Observe the reaction and record your notes 9- Do the same with barium chloride and observe the reaction 10- Pour out the contents in the test tubes and wash them out 11- Pack up your materials Results-Flame test Cation Flame Test A= Calcium Vibrant orange color with a hint of yellow Calcium B=Copper Forest green color with a hint of orange Copper Results- Precipitate Test- Anion Halide Test (Silver Nitrate) Sulfate Test (Barium Chloride) Unknown A Turned cloud white color, has a quick reaction, sediment on the top Clear solution with a white stringy bits floating
Purpose The purpose of this experiment was to evaluate the stoichiometric relationship between the testing agents and to identify the products formed. The relationship was found by completing three acid and base neutralization reactions using phosphoric acid, which is a triprotic acid, with different volumes of sodium hydroxide. Introduction Procedure Phosphoric acid solution with a volume of 1.00 mL and a molarity of 6.00 M was transferred into a 125-mL Erlenmeyer flask using a volumetric pipette. Sodium hydroxide solution with a volume of 6.00 mL and a molarity of 3.00 M was transferred into a 50 mL beaker using a volumetric pipette. While swirling the phosphoric acid solution in the Erlenmeyer flask, the sodium hydroxide solution was added to it a few drops at a time using a disposable plastic pipette.
This procedure occurred in the presence a Bunsen burner. The inoculation needle were placed within the open flame 15-20 second in order to sterilize the needle and prevent contamination. The needle was allowed to cool 5-10 second before inoculation. Using aseptic transfer technique the needle was used to gather up some of the colonies on the plate, making sure not to touch anything else with the needle. The test tube was uncapped and the lips of the test tube was passed through the open flame three times.
Methanol was filled in a test tube and placed into a water bath to heat up. 12 Drops of the Methanol were then added to each flask until the crude caffeine had completely dissolved. 13. The solution was then filtered and the residue collected in a filter paper. It was left to dry and
Using two test tubes, label one “s” for substrate and the other “e” for enzyme. The substrate tube should contain 7 mL of distilled water, 0.3 mL of hydrogen peroxide, and 0.2 mL guaiacol and the enzyme tube should contain 6 mL of distilled water and 1.5 mL of peroxidase. Combine the materials of the substrate and enzyme tubes, mix the two using a clean transfer pipette, transfer a portion into a cuvette so that the cuvette is about half-full then cover the top of the cuvette with Parafilm and then place it in the spectrophotometer and record absorbance. Remove the cuvette and repeat recording absorbance at 1, 2, 3, and 4 minutes. Be sure to mix the cuvette and clean its surface with Kimwipes before each reading.
Next, transfer the sample to a 50 mL beaker and mixes with distilled water, which gets by rinsing the crucible with its cover in 8mL, so the solution is generated. After that, put an aluminum wire into the beaker, and after a certain period of time the solution gains color. To finish the reaction, 5 drops of 6M of Hydrochloric acid is added into the beaker to clean the solution, which means that acid dissolves all salts of aluminum that is on the solution. After finishing the chemical process, collect and use the Butcher funnel to wash the cooper because it is going to be used to a vacuum filtration. After finishing the filtration, measure the weight of the sample and dry it.