Finally the hexane layer was washed with 100 ml water and then evaporated to dryness with a vacuum rotary evaporator. The concentrated extract was then passed through a chromatographic column (30 cm x 10 mm i.d) containing 2 g florisil (lower) and 1 g sodium sulphate (upper) which is pre wetted with hexane: acetone (1:1). OCPs were eluted with 25 ml hexane: acetone (1:1).The solvent was evaporated using rotary evaporator and final volume was adjusted to 5 ml, which is used for GC analysis. All the sediments were analyzed for HCH and
Standard solution The standard solution was tested for its value and was used in the calculation of the patient's urea and creatinine value. ii. Running patient's specimen a. Creatinine 1ml of creatinine reagent 1 and 200ml of creatinine reagent 2 was pipetted into the same 1ml cuvette. Following that, 40ml of patient's sample was transferred into the cuvette. The absorbance of the mixture was measured at 30s (A1), 5min and 6min intervals.
The NaH2PO4 and Na2HPO4 powdered were weighted by using weighing machine, followed the mass that has been calculated in step (3). The NaH2PO4 and Na2HPO4 powdered were mix in a 500 mL beaker. 500 mL of distilled water were measured by using a 500 mL measuring cylinder, then is poured inside the 500 mL beaker containing both the powdered. The mixture were stirred by using a glass rod until the mixture is fully dissolved. The solution were tested by using calibrated pH meter to get the pH value of the solution.
Then five millilitres of sample “A” were placed in the test tube labeled “A”. This was then repeated with the next three samples. Each sample was visually observed and the colour of each was recorded. Next 20 drops of Benedict’s solution were added to each test tube and the test tubes were lowered into a hot bath at a temperature of approximately 80 degrees Celsius. All colour changes were recorded.
To make the 2M HCl solution, 200mL deionized water was added to a 600mL beaker labelled “2M HCl” by using a graduated cylinder. Then, 100mL 6M HCl was added to the same beaker also by using a graduated cylinder. The solution was stirred with a stirring rod. To make the 2M NaOH solution, 50mL deionized water was added to a 400mL beaker labelled “2M NaOH”. Then, 100mL 3M NaOH was added to the same beaker.
First, 50 mL of the sample was placed into a 250 mL Erlenmeyer flask, and onto a stirring plate. Then, the pH of the solution was measured and adjusted to be within the range of 4 and 6, using nitric acid and sodium hydroxide. After the pH was optimal for the experiment, a single mL of indicator- acidifier reagent was added to the sample. Then, 50 mL of mercuric nitrate was place into a burette and titrated with the sample until the color of the solution turned from blue to purple. The volume of titrant used for the reaction to reach endpoint was recorded.
Another 5-mL test tube, labelled as B, was filled with 1 mL of distilled water. A drop of methyl red was added. Also, a 0.01M hydrochloric acid (HCl) was added in a dropwise manner from a syringe until the color of the solution matches that of the first test tube setup. The volume of the HCl used was recorded for the determination of the ionization constant of
In addition, phenolphthalein was added as an indicator. The aliquots were titrated against sodium hydroxide (NaOH) solution until end point was reached, after which volume of NaOH consumed was recorded. The value of the rate constant, k, obtained was 0.0002 s-1. The experiment was then repeated with 40/60 V/V isopropanol/water mixture and a larger value of k = 0.0007 s-1 was obtained. We concluded that the rate of hydrolysis of (CH3)3CCl is directly proportional to water content in the solvent mixture.
In the next steps the density of water between 30-40 °C, 40-50 °C and 50-60 °C was measured. Then our results ρ vs T and also density vs temperature values given in the Steam Tables were plotted on the same graph in order to compare. In the second part the density of water was measured by density bottle. The densities obtained from the experiment are 995, 992.5, 991, 990 kg/m3 for the first part and
Preparation of ketoconazole loaded Proliposomes Ketoconazole (KTZ) loaded proliposomal gel formulations were formulated by method reported by Perret et al. 1991 with slight modification. Constant amount of drug was added to varying ratios of phophatidylcholine and cholesterol which constitute lipid component of 1mmol quantity. This lipid mixture was prepared in clean and dry, wide mouthed glass vials to which 400µL of absolute alcohol was added and after confirming the formation of homogenous dispersion, the glass vials were heated thermostatically on a water bath at 60-70oC with intermittent shaking. Add 160µL of double distilled water maintained at the same temperature to the transparent solutions formed, these upon cooling change to yellow translucent liquid/gel or white creamy proliposomal gel.