1-Introduction
Antimicrobial resistance (AMR) is resistance of a microorganism to an antimicrobial medicine to which it was previously sensitive. AMR is one of the major problem today as well as in the past which causes the major health problem. AMR occur when antibiotics lost the ability to control the growth of microorganism so that they continue to multiply. Some bacteria are naturally resistant while the other may get this ability of resistance either by the gene mutation (which changes the genetic material of the bacteria) or by acquiring the resistance from other bacterium (by the process of conjugation which transfer the genetic material along with the coding gene resistant to antibiotics).
The discovery of the antibiotics was a significant change in the human history as the antibiotics bring change in the field of medicine in many respects and many lives have been saved. The presence of antibiotics without any negative coefficient has changed the public health and this has been the reason of countless improvement in medical care (Spellberg et al., 2008). The resistance of microorganisms have unfavorable effects on human health and the result includes the infections that may increases the chance of failure of
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These samples were diluted in the 0.9% saline solution. Mannitol salt agar (MSA) media was prepared by adding MSA and sufficient distilled water was added to make the media 1 liter. This media was autoclaved at 120 degree centigrade. The media was cooled for some time and then it was ready to make plates. Bacteria soil samples were spread on the prepared cultural plates with the help of cultural swabs. Kept these inoculated cultural plates in the incubator at 38⁰ C for 24 hours. After 24 hours bacterial colonies were seen on the cultural plates. Colour of these colonies was yellow so that indicate the presence of Staphylococus on the
After lawn inoculating a Meuller Hinton plate and placing the samples of medication, the plate was then incubated for one week at 37 degrees Celsius. The first medication choice was Trimethoprim, this produced a zone of inhibition of 16mm, therefore being sensitive to the bacteria. Antibiotic number two was nalidixic acid, this too, has a zone of inhibition of 16mm but is considered intermediate. The next antibiotic was erythromycin which produced a zone of inhibition of zero and was therefore resistant. The last antibiotic that was chosen to be used in the experiment was ciprofloxacin.
After 5 days the plates were removed from the cold room and the gram-negative test for Colony A on the EMB agar showed pink fisheye colonies which lead to the conclusion that the gram-negative organism within Unknown #21 was Enterobacter aerogenes. Had the pigmentation been metallic green, the organism would have been identified as Escherichia coli, and had there been no pigmentation at all a Triple Sugar Iron agar (TSI) test among other tests would have been
Mannitol Salt Agar (MSA) plate, MacConkey agar (MC) plate, Eosin Methylene Blue agar (EMB), and Hektoen Enteric Agar (HEA) (3). The MacConkey agar plate and the Mannitol Salt agar plate are both used in the identification of the unknown. The MC plate is a selective and differential medium. It is considered a selective medium because the bile salts and crystal violet aspect of the medium prevent the growth of gram positive bacteria (3). This medium is differential because of the lactose and neutral red.
I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided
“Antibiotic sensitivity tests showed that the two groups differed in sensitivity to penicillin and erythromycin, but no other antibiotics.” (Article abstract).Out of the nine hundred samples they took, the group showed the bacteria was defeated by the antibiotic penicillin and the antibiotic erythromycin but the other antibiotics didn’t touch the bacteria. The experiment showed people only two antibiotics were fighting off the bacteria. The two of which are being over used and over time will no longer work. If the bacteria continues to grow bigger and better, the antibiotics for ear infections aren’t going to work which leads to hearing loss, even to the extent of going
PHAR 100 Assignment 3 1. Antibiotics are a form of medicine that seek out and destroy the bacteria that make us feel sick. Antibiotics work great against bacteria, however they don’t work against viruses. Penicillin was the first antibiotic to be discovered by Alexander Fleming, and it was first used to treat infections. Essentially, these powerful medicines fight bacterial infections, and have the potential to save lives.
A starch agar plate was inoculated with a streak of the unknown bacteria and then incubated. On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids. The smoke of the Iodine stained the plate to display the presence or absence of a halo around the bacteria 2.12 Lipid Hydrolysis This test was done by making a single line streak inoculation on a tributyrin agar plate and allowing incubation. After the incubation period, the plate was observed for the presence or absence of a halo around the bacteria.
In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
The Spores That Changed the World “The discovery of penicillin not only revolutionized medicine, but changed the world” (“Penicillin”). Penicillin was the first in a class of drugs called antibiotics. The introduction of penicillin ended centuries of failure to cure infections (“Penicillin”). Alexander Fleming is, amongst others, the most notable founder of penicillin. During his summer vacation, Fleming went to his trial lab at Saint Mary’s Hospital to check on some of his ongoing projects.
Antibiotic resistance occurs when bacteria reformat themselves in order to become unaffected by the drugs or substances created to terminate them (About Antimicrobial Resistance). They gain resistance to the drugs which causes the drugs to be ineffective of use and leads to more harm. Antibiotic resistance commonly occurs within patients because of physicians misusing or overusing antibiotics. This creates a common resistance to patients and resulting in the constant creation for new antibiotics that would treat the bacteria (Antibiotic Resistance). The main conflict generally evolves from medical stewardship where physicians have the responsibility to optimally heal their patients to their best efforts (Medical Ethics and the Stewardship
It is highly virulent with the high rate of resistance to the treatment and antimicrobial infectious agents. The mentioned above proves that the Epidemiology of Staph. Auerus as foodborne pathogen requires
After a gram stain was done unknown #257 was identified as a gram positive organism because when observed under the microscope the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with a heavy quadrant one.
Amid Alexander Fleming 's Nobel discourse he said, "After I finished my research, biochemist Dorothy Crawfort Hodkin used x-rays to determine the atomic structure and molecular shape of penicillin. " (Fleming,2)Dr. C.G. Paine, dermatologist Albert Nutt were two famous doctors who used Hodkin’s research to develop several startling cures with the new substance as early as 1930 (Krebs,23) All of the fresh out of the box new research and the newfound properties of penicillin brought these new anti biotics which cured maladies that were very common, which balanced out various sorts of microbes. The life expectancy of the urban population increased by 3 years.
INTRODUCTION: In this experiment I was testing for antimicrobial sensitivity of Staphylococcus epidermidis by using the Kirby-Bauer Diffusion test. The three antibiotics utilized in this lab were: gentamicin, novobiocin, and penicillin. I determined the effectiveness of the antibiotic by observing and measuring the zone of inhibition for each antibiotic.