Essay On Culture Media

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Seven different types of culture media were prepared to subculture both inbred wild type and scarlet eye D. melanogaster. Among the seven types of culture media, five of it are adding different type of sugar in each banana medium whereas another two of media are the control media. Banana (Musa acuminata Colla)) medium was used as positive control while plain agar medium was used as a negative control in this experiment.

Brand of Aldrich Chemistry of powder-type agar was used in the preparation of the culture media. For the step of preparing agar medium, first measured 100 mL of distilled water and boiled it around 50°C by the way also weighted 4.0 g of agar powder and added in the 50°C distilled water and let it fully dissolved. The liquefied agar was used as negative control medium (plain medium) or added with new component to form other culture media. For the making of banana medium (positive control), the banana was weighted as equal quantity of distilled
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For the step of preparing agar medium, first measured 100 mL of distilled water and boiled it around 50°C by the way also weighted 4.0 g of agar powder and added in the 50°C distilled water and let it fully dissolved. The liquefied agar was used as negative control medium (plain medium) or added with new component to form other culture media. For the making of banana medium (positive control), the banana was weighted as equal quantity of distilled water was taken. Next, the banana was blended with some distilled water and then mixed with the liquefied agar and 1.0 g of antifungal chemical. For the different types of sugar banana media, the sugar was cooked until 100% concentration. Then, 5 mL of concentrated sugar was measure and top up to 100 mL with the banana medium. The five types of sugar was used in preparing the sugar banana media were white sugar, rock sugar, honey rock sugar, palm sugar and brown

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