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Essay On Microbiology

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Microbiology is the study of microorganisms (or microbes), which include bacteria, viruses, fungi, parasites, and even prions . In short, microbiology refers to the study of life and organisms that are too small to be seen with the naked eye. Microorganisms are found in almost every habitat present in nature and are vital to humans and the environment. While some microbes are harmful, causing diseases that harm and kill people, animals, and plants, they are exploited by researchers. They have uses in food, water treatment, science and medicine, energy, warfare, and much more.

Conventional microbial analyses require extensive manual operations. In addition, the necessity for visual determination of the growth of microbial colonies leads …show more content…

This reaction can be revealed using a variety of procedures, including A-direct and indirect immunofluorescent microscopy assays (IFA), B- flow cytometry, C- enzyme-linked immunoabsorben D-Latex agglutination
A- Immunofloresence Assay (IFA): Direct & Indirect Direct IFA: AB conjugated with Fluorescein marker + Bacteria ( Antigen) = Immuno complex Indirect IFA: Primary AB + Bacteria= Immune Complex+ Secondary Fl conjugated AB B- Flow Cytometry rapid identification of oral bacteria involves labelling bacterial cells from a patient plaque sample with both species-specific antibody and a second fluorescein-conjugated antibody. The suspension is then introduced into the flow cytometer, which separates the bacterial cells into an almost single-cell suspension by means of a laminar flow through a narrow tube. The sophistication and cost involved in this procedure precludes its wide usage
Bacterial cells+ species specic AB + Secondary FL Conjugated AB --->Introduced in flowcytometer
# Bacterial cells is separated into single cell suspension passes through the tube---> Cells identified by lasers.
C- (ELISA) Enzyme Linked Immunosorbent Assay

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