Essay On Micropipettes

Azarbad
15701821
‘How to use a micropipette’
Aim
To be able to use micropipettes in an appropriate way in order to measure and transfer small volumes of liquids.
Introduction
Micropipette is a usual laboratory equipment used to transmit a measured volume of liquids. Remarkable 19th century chemist and microbiologist, Louis Pasteur, invented the first pipettes which were made of long, thin, glass tubes in order to ensure that liquids are transformed between different tubes without entering the external environment and being contaminated. These pipettes helped many important discoveries during that time. However, their friability was a great issue to concern. Therefore, the plastic micropipettes were invented by Heinrich Schnitger, a post-doc in the University of Marburg, Germany in 1957. [1] [2]
Micropipettes are used in numerous laboratories such as microbiology, medical, environmental sciences and research labs and have major parts in many routine tasks, for instance, volume dependent experiments, quantitative measurements and distributing analytical samples. [3]

But each micropipette has some essential parts including Volume adjuster knob which is used to adjust the volume of the desired liquid, Plunger button placed under the volume adjuster and is pressed to pick up and distribute the liquid in the micropipette, Tip ejector button is for releasing the different tips of each transfer, Digital volume indicator which shows the volume of the liquid, Ejector arm, Plastic shaft and finally the Tip attachment where the disposable tip is located in and is in the bottom of the pipette and tips are used only for one transfer and should be discarded into a suitable receptacle after the completion of the transfer.