Essay On Spectrophotometry
Spectroscopy is an area involved in chemistry, where throughout history has been used for the analysis of a whole wide range of things from the analysis of food content to pharmaceuticals. Spectroscopy is the interactions between matter; meaning molecules and atoms and radiation such as infrared radiation5. MRI scans to acquire a through image of the human body for disease diagnostics is an example of spectroscopy. Which is the interaction of hydrogen atoms with radiowaves5. As for food dyes, which is typically used in commonly consumed products such as Gatorade and many commercial drinks.
The appearance in color is the result of interactions between the substance and the specific section in the electromagnetic field that is “visible”5. The
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Each substance was measured and weighed to 0.5g and Yellow 6 was measured and weighed at 0.1g of dye. And were diluted with 30ml of distilled water and placed in a separate flask and labeled as stock solution. Stock solutions were then serial dilutions of stock solutions were done by using 10ml of stock solutions were measured by a graduate cylinder and placed in separate 100ml volumetric flask and distilled water was added to volumetric flask to dilute by a factor of 100ml. then get sample of the second stock solution using a dropper to add drops of solution to cuvette up to the line marked. Then it is placed into slot in the spectrophotometer to analyze the absorbance and wavelength. The absorbance rate has to be between 0.1-1.0(OD). If this rate is not achieved continue serial solutions. Then once this rate is achieve record the lambda max and absorbance. Then after rate is achieved record five points of concentrations by further serial dilutions of stock solutions. After first week of experimentation commercial product was brought in and 10ml of Fruit Water was used with 90ml of distilled water using volumetric flask to make of stock solution and was put into the spectrophotometer to read absorbance and lambda
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