Hydrocarbon Lab Report

Role of Microbes in Hydrocarbon Degradation

 INDEX

• Introduction

• Rationale of Studies

• Objective of the experiment

• Materials and Methods

• Factors Affecting Microbial Hydrocarbon Degradation Activity

• Result and Discussion

• Reference

• Introduction –

Hydrocarbon enter in environment accidently or due to human activity. It affects both marine and terrestrial ecosystem. They are some micro-organism which survive this due to their different enzymatic and physiological response. Hydrocarbon are the pollutants that cause mutation and sometime may be death when enter in environment. We can use different technology to remove hydrocarbons like burying, evaporation, dispersion and washing but these methods can be very costly, so we use microbes for this purpose. There

But for the ease of experiment we will use naphthalene as an energy source for hydrocarbon degrading bacteria.

• Chemicals and growth media –

• Lennox broth for Escherichia coli{1}

• Naphthalene’s main carbon source

• Nutrient agar and liquid king B media for pseudomonas strain {1}

• Microbial isolation and growth conditions-

• Soil samples are collected from oil contaminated soil samples from various spotsand isolated by pour plate method.

• Morphological recognition-

• The Microorganism are grown on various media for a few days at a certain temperaturethey were identified as pseudomonas aeruginosa, bacillus spharicus and Escherichia coli .

• For Bacterial isolation media is required such as “Bushnell-Hans medium” along with some amount of glucose solution. Bushnell-Hans media has following composition

- KH2PO4 (0.4g)

- K2HPO4 (0.4g)

- (NH4)2SO4 (0.4g) or

- NH4NO3 (0.4g)

• Inorganic Nutrients are required to fulfill the extra nutritional need of bacteria. These inorganic nutrients are – Nitrogen