Introduction: The objective of this experiment was to use a spectrophotometer to calculate the absorbance wavelength for the commercial dyes that were given. Light is composed of tiny particles that are called photons, just like matter is composed of tiny particles called atoms. Using the spectrophotometer you can see that different dyes absorb at different wavelengths. With all of the experimentation done the concentration, absorbance and the max wavelengths should be found. Figure 1: Schematic of a single beam UV spectrophotometer Materials: There was an array of materials needed to complete this experiment.
Contrast i. Wavelength of radiation The range of visible light is 400-700nm. Different rays have different wavelengths as shown in fig 1. So, before the experimentation one should know the specific wavelength of a particular radiation ii. Light refraction and image magnification Light is refracted when it enters from a medium of light into glass. When a light bends while passing through a lens, then light rays are focused at a point that particular point is known as Focal point and due to which a larger image of the object is observed.
Based on the Beer-Lambert Law, the absorbance of light of a sample is directly proportional to the concentration of the molecule which absorbs light. Distilled water is used as a blank solution to set the spectrometer to a zero reading due to none of the absorbing species present in the solution tested. In this experiment, heat is used to speed up the browning of reducing sugar to produce furan. It is due to the chemical reaction of the reducing sugar with the free amino group of an amino acid. The amino group that is present in monosaccharides sugar are a short chain, therefore it is more reactive.
Microspectography Microspectography is a technique highly specialized for the measurement of spectra. Fluorescing dyes sometimes show a different spectral behavior in the unbound state found in solution in cuvettes, and the bound state to cellular components. Spectral characteristics are determined by combining a fluorescence microscope with a spectrofluorometer. This instrument detects the variance of the adsorption wavelength and the variance of the emission wavelength 4.4. Quantitative fluorescence microscopy Quantitative fluorescence microscopy involves taking measurements from fluorescing specimens.
First let’s explain what Ultra violet radiation is. Ultraviolet radiation definition is radiation in the part of the electromagnetic spectrum where wavelengths are just shorter than those of ordinary, visible violet light but longer than those of x-rays. In other words it’s dangerous to play with. It is unstable molecules known as reactive oxygen species. (Source: Journal of Aesthetic Nursing (J AESTHETIC NURS), Jul/Aug2015; 4(6): 276-280.
oduction Spectroscopy is the interaction between the electromagnetic radiation and the matter, which results in different spectra. These spectra are useful to detect functional information about the chemical compounds. Several sources o regions are involved in spectroscopy in which one involves the use of infrared region that ranges from 400- 800nm. The infrared spectroscopy interest in the fundamental vibrations of the molecules caused with the interaction of IR radiation. IR spectra is used to identify the functional groups in the compounds as each bond has a different tendency to vibrate.
Beer concluded that the absorbance of the light by the substance is proportional to the concentration of the substance in the sample. The modern definition of absorbance correlates the Beer-Lambert law as a function of concentration to the path length of the light travelled through
The influence of ultra violet rays in life Introduction The source of natural UV radiation is the sun, but due to the absorption of oxygen atoms to form the ozone layer, the lower-intensity terrestrial solar radiation that includes the UV with a wavelength of 290 -400 nm, while the shorter wavelength is absorbed by the atmosphere. As a major absorber of UV radiation, this gas layer serves as the protector of the earth from exposure as UV radiation shorter than 340 nm. The reduced ozone layer as a result of the release of human-made chlorofluorocarbons (CFCs) into the atmosphere will reduce the level of ozone protection against UV light and cause the level of damage due to UV radiation exposure is greater. UV rays are electromagnetic that lies supine in the 100 nm-400nm wavelength range divided into ultraviolet A or UV-A (λ320-400 nm), UV-B (λ280-320 nm) and UV-C (λ100- 280 nm). UV rays have a bad impact
=c/ Wavelength indirectly proportional to energy. The condition in which light is utilised in raman scattering and infrared absorption spectroscopies is quite different. In infrared absorption the analyte is exposed to a radiation of a broad range of wavelengths and when the specific incident light frequency matches the vibrational energy level of the analyte, the molecule is stimulated to a higher vibrational state. It is analysed the loss of energy of the incident radiation due to these absorptions. On the other hand, Raman scattering adopt one unique incident radiation at a fix wavelength to interact with the sample material.