Ethephon Production

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In the control fruit, ethylene production increased gradually throughout the 12-day period at 25ºC (Fig. 1A). Ethephon treatment induced an earlier onset of ethylene production, with a large effect during day 1-3. In contrast, 1-MCP treatment significantly delayed ethylene production until day 5 compared to the control fruit and ethephon treatment (Fig. 1A). For control fruit, durian pulp firmness rapidly decreased by day 3 during ripening at 25 °C. (Fig. 1B). Using ethephon-treated fruit, pulp firmness decreased more rapidly than the control fruit. 1-MCP treatment delayed pulp firmness decrease for 9 days of ripening at 25ºC. However, a rapid decrease in firmness was found in fruit on day 12 and 15 similar to the rate found in the control …show more content…

Gene expression in the pulp Gene expression of DzACS1 and DzACS2 in control durian pulp increased significantly to a maximum on day 7 and 5 during fruit ripening, respectively. Ethephon treatment was found to induce DzACS1 and DzACS2 gene expression only on day 5 and 3 respectively, compared to the control durian pulp (Fig. 3). 1-MCP treatment inhibited strongly the expression of both DzACS1and DzACS2. The effect of 1-MCP inhibition on both DzACS1 and DzACS2 was not apparent from day 9 onwards (Fig. 3). The transcript abundance of DzETR1, DzETR2, DzCTR1, DzEIL1 and DzEIL2 were high in the control durian pulp during fruit ripening on day 1-7 (Fig. 4 and 5), with the highest expression of DzETR2 (Fig. 4B) and the lowest of DzEIL1 (Fig. 5B). An increase in DzETR1 and DzETR2 expression in control fruit reached a maximum on day 7 and 5, respectively and decreased sharply thereafter. Ethephon treatment increased both DzETR1 (Fig. 4A), DzETR2 (Fig. 4B) and DzCTR1 (Fig. 5A) expression to a maximum on day 5 and decreased thereafter. 1-MCP treatment severely reduced the expression of all five ethylene receptor and transduction genes. This expression then gradually increased from day 9 onwards (Fig. 4 and …show more content…

Fig. 1B). Predicted TATA-box and CAAT-box of the core promoter were identified. The important regulatory cis-acting element found was a binding site for ethylene response factor 1 (aTGTATttc). Other cis-acting elements identified included a light responsive element (3-AF1; TAAGAGAGGAA), an anaerobic induction element (ARE-box; TGGTTT), a gibberellin-responsive element (GARE-motif; AAACAGA), a low temperature responsive element (LTR-box; CCGAAA), a stress responsive element (TC-rich repeats; GTTTTCTTAC) and a salicylic acid responsive element

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