3.1. Materials and microorganisms Eugenol (99 %); Coniferyl Aldehyde (98 %); Ferulic Acid (99 %); Vanillin (99 %); Vanillic Acid (99 %) were obtained from Sigma Aldrich. Methanol used for high-performance liquid chromatography (HPLC) analysis was purchased from Merck and was HPLC grade. All the other chemicals used were of analytical grade and commercially available. The Pseudomonas fluorescens NCIM 2100 bacterial strain was obtained from National Collection of Industrial Microorganisms (NCIM), Pune and was provided by Department of Bio-Engineering, BIT Mesra. Methodology for screening After subculturing and maintenance of Pseudomonas fluorescens NCIM 2100, obtained from departmental culture stock, Starter bacterial culture of known microorganisms …show more content…
Medium and culture conditions Cells were grown in a 100-mL flask containing 25 mL into minimal medium supplemented with Eugenol for 2, 4 and 6 days incubation at 37 °C. The following Table 3. represents the composition of the modified minimal media used for biotransformation of eugenol (Muheim and Lerch, 1999). The pH of the medium was adjusted between 7.0 to 7.25 and autoclaved to obtain sterilized media for further …show more content…
Effect of Various nitrogen sources: The modified minimal media used for biotransformation of Eugenol has yeast extract (0.5gm/L) as its sole nitrogen source. This was replaced by beef extract, peptone, ammonium nitrate and sodium nitrate respectively at 0.5gm/L to monitor effect of various organic and inorganic nitrogen sources. Culture was kept under optimum temperature and pH for an incubation period of 2, 4, and 6 days. 3.5.5. Effect of varying temperature and pH: Effect of varying temperature (5 °C, 25 °C, 37 °C and 45 °C) on biotransformation of Eugenol was studied at pH of 7. After choosing an optimum temperature, pH was optimized in the range of 5-9 to observe its effect on production of valuable products. Similarly, Medium and culture conditions were maintained as described in section 4.2. The cultures were incubated for 2, 4, and 6 days to monitor effect of varying temperature and pH on production of different metabolites during biotransformation. 3.5.6. Effect of inoculum size (v/v %): Effect of different inoculum size (2 %, 3 %, 4 %, 5 % and 6 %) on production of desired metabolites by the selected microorganism was studied. The modified minimal media used for biotransformation of optimized Eugenol and other different parameters was studied for the effect of different inoculum size (v/v %). Culture was kept under optimum temperature and pH for incubation period of 2, 4 and 6
Methods Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398. The identification of unknown #398 followed test procedures from Brown1.
12/10/2015: Eugenia Clement, RD, LD request that I speak with the mother of her 3 year old patient, De Breia Oliver, a 23 year old African American, married mother and self reported to being depressed. Ms. Oliver states that she just works and sleeps and finds little pleasure in doing anything else, she has very little energy and although she gets hungry she doesn’t eat much, but this she states is not impacting her Diabetes I (although Eugenia reports that she was told that it is impacting her Diabetes). Ms. Oliver scored a 15 on the PHQ-9, indicating that she is moderately severely depressed. Eugenia Clement is concerned that her depression is impacting her choices in foods for her daughter (she is reported to eat primarily fast food). Ms.
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided
Introduction Our world is composed of many bacteria’s’ that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods that have been learned in the laboratory to determine our unknown bacteria. Bacteria’s can have different features, shapes, and or arrangements that help microbiologist determined their role in our life (whether they are good or bad for humans).
Another hypothesis made was that the bacteria would glow with the addition of the sugar arabinose. All three of the objectives seem to go hand in hand. The lab began by inserting transformation
Materials and Methods To start with, the unknown bacteria # 710 broth had to be successfully isolated on an EMB and MAC agar plate. Using aseptic technique by sterilizing the wire loop with Bunsen Burner between inoculations and flaming the opening of the test tubes before inserting in the loop with the bacteria. The streaking technique used was to isolate the colonies on the agar plates. In addition, the streak plates had to be incubated in a upturned position for 24 hours in a hot temperature incubator at 37 degree Celsius. Bacteria need a favorable condition to grow in.
Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1.
- Little to no oxygen will result in the E. coli cells producing large quantities of acetic acid, causing the growth medium to reach pH 4 or lower however, with proper aeration the cells will be able to use many organic acids as carbon sources and the pH of the growth medium will be maintained at neutral or basic ranges. Aeration is another important factor in determining E. coli cell growth however it can continue to grow in the absence of oxygen using fermentation and anaerobic respiration. - Optimal growth temperature is 37C, cannot grow well at temperatures higher than 42C and they can tolerate lower temperatures with lower growth rate. - The doubling time or generation time for most E. coli strain in a rich medium at 37C is 20 minutes.
Saint Eugenia was born in Rome, Italy, in the year of 183 A.D. Her father, Philip, was the governor of Egypt chosen by the emperor Commodore. Eugenia and her family lived in Alexandria. At that time, the Christians had been driven out of Alexandria and were living outside the town. (Saint Eugenia Orthodox Church - Events) Eugenia received an excellent and complete education because her family was rich.
Daniel Lee Mr.Mike 20 January 2016 Pseudomonas putidu Everyone thinks that bacteria is harmful to us because they usually think that bacteria is like a disease. But actually it is not. Bacteria is helpful to us.
3.5. Evaluation of Pseudomonas spp. as plant growth-promoting bacteria under salt stress Four strains, Pseudomonas plecoglossicida, strain 24, Pseudomonas sp., strain 30, P. putida, strain 103 and P. fluorescens, strain 109 which are salt tolerant and have the following plant growth-beneficial traits: IAA production, phosphate solubilization, and ACC deaminase production were selected for maize growth stimulation under salinated soil. For non-saline soil (NSS) treatment, strain 103, characterized as P. putida, was able to significantly increase plant development for all the analyzed parameters, except for root dry weight.
In vitro investigations have been done on the genotoxic effects of zinc oxide eugenol and resin based sealers used in dentistry for root canal. Authors have suggested a moderate to severe toxic effects of zinc oxide eugenol in V79 cell line and also demonstrated that these effects are dose dependent suggesting that eugenol has cytotoxic but not genotoxic effects (41). On the other hand the chemopreventive effect of eugenol on DNA damage induced by 7, 12 dimethylbenzanthracene (DMBA) has been evaluated in MCF-7 cells. The observations suggested that eugenol was potent to protect DNA against genotoxic damage induced by DMBA. Eugenol is able to suppress the DMBA activation and acts as a potential chemopreventive compound
Materials All the chemicals used for synthesis purposes were of AR grade, purchased from Sigma Aldrich chemicals, and for spectral analysis spectral grade solvents were used. Double distilled water was used for preparing solutions. The test strains, Escherichia coli (E. coli) gram-negative, Staphylococcus aureus (S. aureus) gram-positive bacteria and Pseudomonas aeruginosa (P. aeruginosa) were purchased from IMTECH, Chandigarh, India. Yeast extract, tryptophan and bacterial-grade agar–agar were purchased from Hi-media Laboratories, Mumbai, India.
[Include any grant/funding information and a complete correspondence address.] The Nature and Importance of Pseudonomas Micro-organisms have come to be of great significance to us human beings. Their uses are plentiful and diverse but a few of the most common applications of these genera are in food production, medicinal research, health, ecology and biotechnology. In this essay I will be talking about the genus pseudomonas in particular. The basic few things to know about the pseudomonas bacteria is that it falls under the class of proteobacterias, belongs to the family of Pseudomonadaceae and is a Gram negative bacteria.