Once this point was reached, the timer was stopped and the time was recorded in Data Table #1. The same steps involving the addition of Na2S2O3 were repeated for Wells #2 and #3, using 2 mL of Na2S2O3 in each. The final times for each well was recorded in Data Table #1 in the appropriate blank. Once the first trial was completed for the first three experiments, a second trial was completed in Wells #4, #5, and #6 using the same procedure in order to increase accuracy. After each trial was conducted, each of the wells were emptied into the designated waste container and dried using a paper
According to EPA government; Recycling Basics; it states that “Recycling is the process of collecting and processing materials that would otherwise be thrown away as trash and turning them into new products.” Recycling has multitudinous profits. One of the biggest reasons why recycling is promoted is; as it reduces the
In Part A of the experiment which is sterilisation technique, three types of technique is used. The first technique is dry-heat sterilisation. Dry heat sterilisation takes a long time and is done at a high temperature of about 160°C for 60 minutes. In dry-heat sterilisation, there is two parts which are heating and flaming. Heating is when the inoculating loop and the needle are burned in the direct flame until it turns red in colour while flaming is just passing the forceps and mouth of the culture tubes through the flame to prevent bacteria from entering.
Formula 2: % Component= 100% component mass (g) sample mass (g) Procedure First, we measured out the evaporating dish to find the mass. Then we added around 3 grams of our sample (2.832g exactly). Next we added the isopropyl alcohol to dissolve the Benzoic Acid. We filled the evaporating dish, stirred, and then decanted the sample into a 140mL beaker with a stirring rod. This
Referring to Table 1, the reactants for each run were transferred to an Erlenmeyer Flask (250 mL) via a buret. Using a precision pipette, the volume of I3- required for each run was carefully extracted and poured into the flask containing all of the reactants. Immediately after the Iodine solution was placed in the flask, the LabQuest began collection data. Meanwhile, a small portion of the solution, was used to rinse the cuvette, then using a disposable pipette a small amount of the solution was transferred to the cuvette (approx. ¾).
Worms A, B and C were then placed into separate containers containing the caffeine treatment solution. After allowing the worms to be treated for fifteen minutes, they were briefly rinsed and placed in the viewing chamber to measure their pulsation rate. This was done three times for each worm to calculate a mean rate for first level of treatment, 3.0 mM of caffeine. The same procedure was repeated for part II of the lab, however; a different set of three worms were treated with nicotine and all the means were collected to calculate the standard deviation of each
The inoculation loop was sterilized in a fire for 5-7 seconds and then cooled to dip into the starting culture for microbes inoculation on the plate. Allowed the inoculation plate to grow in a constant 32℃ temperature incubator for another 16 hours. After 16 hours, the plate we streaked had shown colonies thinning with the streaking direction
We took 3 test tubes and added 3 ml ammonium sulphate broth and autoclaved them. Then labelled them as +ve control, -ve control and test. In +ve control, we added 0.1 gram soil, in –ve control,we didn’t add anything and in 3rd test tube we added 0.1ml from mother culture. We incubated all of them at 37°C for 7 days. We took slide and divided it into 3 portions and labelled as +ve, -ve and test.
Loaded lanes 1-7 with our 30μl of each of our samples, using a fresh micropipette tip after each use. Recorded which sample was in each lane. Put lid on electrophoresis chamber and connected the leads to the correct power supply. Turned on power and set voltage to 100 volts. Ms. Lovrien turned power off after a set amount of time (Checked to see if bubbles were rising from negative and positive wires).
Now we are talking about recycling. So, what actually does recycling means? Turning used materials that are labeled as recyclable over to your local waste facility designated in a disposal container as “recyclable” materials to be taken and reused as material for a new purpose defines recycling . In order to create a new and different product, a recyclable product is turned back into a raw form that can be used. Recycling efforts can significantly reduce additional waste that will not only harm the planet today , but future generations as well.