We then slowly added 25ml of chilled deionised water to the filtrate to initiate crystallization by using a measuring cylinder and a dropping pipette, once we had done this we left it for about 10 minutes to allow crystallization at room temperature. We then weighed a filter paper which we will use later in the experiment. We then collected the crystallized acetylsalicylic acid by vacuum filtration in a Buchner funnel and washed the product with a little ice-cold water. We then pre-weighed a clean, empty watch glass and labelled it with our initials and the date, we did this do we could easily identify that it was ours when we go to weigh it with the crystals on. We
Once 3-4 readings for the solution were collected, the 2M NaOH was added to the solution. The lid was quickly replaced in order to prevent heat from escaping and not being recorded by the temperature probe. The cup was swirled until the temperature reached a peak and began decreasing. After the 180s had passed, data collection ended. The solution was discarded into the waste bin, and the materials were washed.
One group member monitored the hot plate, while a second group member retrieved a stirring stick to stir in the beaker, this was to help the copper sulfate to dissolve. Two different group members observed what was occurring so that notes could be taken. The final group member retrieved a small crystal, a roll of string, scissors, and two paperclips from the lab table at the front of the room. They brought the items back to a lab table adjacent to the one that the experiment was being conducted at. There the group member cut off string and returned the roll to the front of
¾). Placed the cuvette sample in the Sprectrovis. After each run, the temperature of each sample was collected (to nearest 0.1°C). Disposed of the sample solution, cleaned the cuvette with DIW and repeated the latter procedure using the correct volumes for each new run from Table 1.
Then an estimated (by trial and error) 1-3 grams of hydrated copper sulfate was added to a crucible with the lid on top. The total mass of the hydrated copper sulfate was recorded by subtracting the total mass of the crucible, lid, and sample from the mass of the crucible and lid (described in table 1.3). By attaching the wire triangle to the ring, the crucible was able to sit securely while having the bunsen burner underneath. Lighting the burner once again, each substance was heated for several minutes until estimated that the compound had changed color. Once a prevalent color change had been observed at approximately 4 minutes (blue green color) the crucible was set on the counter using the tongs to cool for approximately 5 minutes.
This study was conducted with a partner, since some parts of the experiment were able to be done simultaneously. One partner prepared a TLC developing jar by pouring a small layer of 4:1:1 propanol/acetic acid/water into a developing jar. A solvent wick was made by wetting a piece of filter with the solvent, and it was placed in the jar. A silica coated TLC plate was obtained, and a spotting line was carefully drawn approximately 1.5 cm from the bottom of the plate using a pencil. Extra care was taken to not touch the plate with bare skin.
Our whole group met I could not make it because of SCAD transportation. I agreed to editing and the rest of the group agreed that I would edit the footage that they filmed. To make sugar glass we had to cook. The ingredients we used for this project are 2 cups of distilled water, 1 cup of light corn syrup, 3 1/2 cups of Sugar, and a 1/4 teaspoon of cream of tarter. First we add all ingredients into the metal cooking pot.
The Incredible Egg The purpose of this lab was to find out what would happen over time to weight of an egg after we scrubbed the eggshell off in a little spot. We then soaked it in water for 10 minutes, 30 minutes, 24 hours and measure how much the weight went up. Once we came back the next day we measured them after 24 hours. In the same day we put the syrup in the cup instead of water and we measured how much it weighed after 10 minutes, 30 minutes and 24 hours. As we did this with the water the weight of the egg went up because the water soaked into the egg and made it heavier.
In this lab, genes for a fluorescent green protein (GFP) and antibacterial resistance (ARG) were inserted into E. coli bacteria. E. coli bacteria was resuspended in an ice-cold CaCl2 solution. DNA containing GFP and ARG was added to half of the cells before they were “heat shocked” in an ice bath and hot water. The heat shocking made the bacteria’s cell membrane more porous, so the DNA could enter. Recovery broth was added to the cell suspension, and the bacteria was placed in warm water for about thirty minutes (see Results and Discussion, paragraph 2).
In this lab, we separated into individual groups to create our modified recipes. We each had two hours, and our ingredients premeasured, allowing for a quick start to our cooking process. Each kitchen had to change a recipe to have a lower fat content as well as, sodium, sugar, carbohydrates or another healthy alternative. After a brief discussion, we began cooking our modified recipes. The recipe I chose to modify was biscuits and cocoa gravy, a sweet treat that is high in sugar content but very filling.
Four randomly selected Daphnia magna, for each trial, were removed from the provided colony for the bioactive compounds to be tested, and were transferred with a plastic wide-mouth pipette with approximately 10 mL of pond water to protect and ensure survival of the Daphnia. In order to acclimatize the Daphnia to laboratory conditions, they were then placed onto a petri dish on the Daphnia cooling chamber. The cooling chamber was located on the stereomicroscope platform and brought down the heart rate of the Daphnia to a range that was countable by the observer, since Daphnia heart rate at room temperature is too rapid. On the cooling chamber there were two petri dishes: one for the Daphnia that were going to be tested, and one with the Daphnia being tested on, to ensure constant consistent temperatures for each trial. To maintain a temperature conducive to the heart
Initially DNA was loaded into well five, however gel was pierced so samples were moved one well to the right. The gel was run at 100 V for one hour. The gel was stopped and observed under a UV illuminator. After the verification of both a single band in the DNA and PCR wells, the PCR product was to be
(1;p 326) Method I collected 1/8 c of saliva, then added the following: 2 drops of dish detergent (stirred 30 sec), 2 drops of contact lens solution (stirred 30 sec), and a small pinch of table salt (stirred 30 sec). Then, I tilted the cup approximately at a 45 degree angle and ran ¼ c of the ice cold rubbing alcohol down the side of the cup, which formed a layer on the top of the saliva-soap solution. (no mixing). Once the solution separated I then took two sticks and wrapped the DNA around them by gently spinning the sticks in the school with the DNA streaks. Final step was mailing the DNA sample to Tory Blackwell for PCR analysis.