The substitution reaction was successful but not fully effective. 19. If the data was inconclusive, then comparing various compounds and the unknown based on physical characteristics would be the first step, titrations would also be a good method. 20. To get a better yield, redoing the experiment would require careful attention in the recrystallization steps: amount of solvent used, how hot solvent is, if the mixture cools to room temperature before placing it in an ice
We had to refill the beaker which most likely caused the ratio of baking soda to water to be lowered. We then successfully finished the trial. We learned that light and the conicity of water are all contributing factors to how fast photosynthesis is done. This experiment was done but it did not turn out well. The whole class ended up redoing the experiment and the results above are those of the second experiment.
The reason California did this was to strictly regulate greenhouse gases such as carbon dioxide. The EPA struck this bill down, yet California is still trying to enforce the carbon dioxide to lessen air pollutants. In California each vehicle after a certain year must pass a smog emission test. If the vehicle does not pass the test it must undergo a certain amount in dollars of repairs to correct the problem. In many cases the vehicle did not pass because of a faulty catalytic converter, or an ill tuned up vehicle.
Introduction In class, a series of experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas. Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were formed.
The urine flow rate would begin to fall due to a lack of solution diffusing into the blood stream; however it should be able to fall for a short time before hitting critical levels. Experimental design and methods: I will perform three tests. The first is my control during which no treatment will be administered. During the second the patient will receive a constant saline drip of 750 mMol/L concentration at a rate of 10 mL/min. during the final test the patient will be administered the same concentration (750mMol/L) at the same rate (10mL/min), however they
It may contain other contaminants like toxic metal salts, hormones and pesticides. Tap water is usually available from the faucet for less than a penny a gallon. Water treatment plants that provide tap water have to be tested multiple times a day. Bottled water is not safer than tap water. More than half of all bottled water comes from tap.
Tap water can contain other substances, therefore might affect the electrical conductivity. However, it probably did not have that much of an effect because they kept the solvent (tap water) controlled throughout the experiment. They did not use tap water for some trials and distilled water for other trials. Something I found difficult in carrying out the method was to make sure that the amount of substance is exactly 10g (or 30g or 60g).
For ascorbate peroxidase assay extraction 241 buffer was supplemented with 1.0 mM ascorbic acid. The homogenate was centrifuged at 242 15,000×g for 15 min at 40C, and the supernatant was used as a crude enzyme extract. 243 Spectrophotometric determinations were performed using UV visible spectrophotometer 244 (UV-1700, Shimadju, Japan). 245 2.11.2. Estimation of superoxide dismutase (SOD) activity 246 SOD activity was estimated by its ability to catalyse NBT to formazan at 560nm 247 according to the method of Beyer and Fridovich (40).
This test tube lacked hydrogen peroxide in order to keep the reaction from occuring. In two test tubes labeled “Substrate”, we mixed 0.2 ml of hydrogen peroxide with 0.1 ml guaiacol and 4.7 ml of distilled water. We also labeled two test tubes “enzyme” using a glass marking pen and filled them with 1.0 ml of turnip extract and 4.0 ml of distilled water. After the test tubes were prepared, we put the blank test tube into a cuvette and put it into the spectrophotometer in order to zero it out. While one group member set the spectrophotometer to zero, another mixed one enzyme test tube with one substrate tube and observed a change in it’s color.
With the increase in use of e-cigarettes among smokers and nonsmokers, academics are pitching their strongly held beliefs for and against its use. These electronic devices that closely resemble a conventional cigarette, and mimic the style of smoking are advancing new concerns over the potential harm vaping may bring. At the turn of the 21st century, smoking rates among teenagers has steadily declined, however with the introduction of the relatively new e-cigarette, this phenomenon is no longer the norm and we see a dramatic increase in e-cig smoking rates. According to a study published in The American Journal of Public Health in December of 2015, Electronic cigarettes are now the most common tobacco product used by adolescents. The electronic
Natalie Koch Bio 2 Metridium The metridium, which is also known as the plumose anemones, is a sea anemone that is classified under the kingdom Animalia and the phylum Cnidaria. Which is the same phylum as jellyfishes and corals. The metridium is typically found in cooler waters of the Pacific and Atlantic oceans up in the Northern Hemisphere (Wikipedia.com, 2013).
Four randomly selected Daphnia magna, for each trial, were removed from the provided colony for the bioactive compounds to be tested, and were transferred with a plastic wide-mouth pipette with approximately 10 mL of pond water to protect and ensure survival of the Daphnia. In order to acclimatize the Daphnia to laboratory conditions, they were then placed onto a petri dish on the Daphnia cooling chamber. The cooling chamber was located on the stereomicroscope platform and brought down the heart rate of the Daphnia to a range that was countable by the observer, since Daphnia heart rate at room temperature is too rapid. On the cooling chamber there were two petri dishes: one for the Daphnia that were going to be tested, and one with the Daphnia being tested on, to ensure constant consistent temperatures for each trial. To maintain a temperature conducive to the heart
One thing I had to make sure that it does not affect the experiment is the light in the microscope. I had to turn it off when the daphnia in on the slide to make sure that the heat doesn 't affect the Daphnia. Every two minutes I go to the microscope to check whether the Daphnia died or