Upon finding the actual concentrations of salicylic acid, concentration of aspirin in the flask at various times can be found using the equation [aspirin]t = [aspirin]0 – [salicylic acid], since at constant volume, number of moles of initial aspirin decrease to form salicylic acid. Initial concentration of aspirin formed as follows: [aspirin]0 = 0.212g / (180.157gmol-1 * 50/1000 L) = 0.0235 mol L-1. Thus using the first test as sample, [aspirin]t = 0.0235 – 9.981*10-4 = 0.0225 mol L-1. To find the rate constant, we will need to log the value of [aspirin]t and plot it against time to find the rate constant. Figure 1 shows the diluted and actual concentrations of salicylic acid, the concentration and log value of aspirin at various times.
The NaH2PO4 and Na2HPO4 powdered were weighted by using weighing machine, followed the mass that has been calculated in step (3). The NaH2PO4 and Na2HPO4 powdered were mix in a 500 mL beaker. 500 mL of distilled water were measured by using a 500 mL measuring cylinder, then is poured inside the 500 mL beaker containing both the powdered. The mixture were stirred by using a glass rod until the mixture is fully dissolved. The solution were tested by using calibrated pH meter to get the pH value of the solution.
The supernatant was assayed for SOD activity by following the inhibition of epinephrine auto-oxidation. 0.5ml of sample was diluted with 0.5 ml of distilled water, to this 0.25 ml ethanol, 0.5 ml of chloroform (all reagents chilled) was added. The mixture was shaken for 1 min and centrifuged at 2000 rpm for 20 min. The enzymatic activity in supernatant was determined. To 0.05 ml of carbonate buffer (0.05 M, pH 10.2) and 0.5 ml of EDTA (0.49 M) was added.
In Section A, the average mass of the 50 ml beaker was weighed 3 times using 2 different electronic balances. The final average mass recorded was 33.73g. The volume of distilled water needed was calculated from the mass of water using the density= 0.9971g/mL in Section B. The volume of water needed was transferred using pipette, graduated cylinder and Erlenmeyer flask respectively. In Section C, a pipette was calibrated by measuring the water temperature and the density was determined.
SYSTEM SUITABILITY THEORETICAL PLATES: A standard solution of 25 µg mL-1of Amoxicillin trihydrate (in triplicate) was prepared and same was injected, then the system suitability parameters were calculated. Theoretical plates per meter Theoretical plates per meter were calculated from the data obtained from the peak using the following expression n = (5.54Vr2)/LWh2 Theoretical plates per column Theoretical plates column were calculated from the data obtained from the peak. n = (5.54Vr2)/Wh2 Where, ‘n’ is number of theoretical plates per meter, ‘Vr’ is the distance along the base line between the point of injection and a perpendicular dropped from the maximum of the peak of interest and ‘Wh’ is the width of the peak of interest at half peak
The mixture is allowed to reach its boiling temperature 110.6°C (383.75K) and mixed by magnetic-stirring bar from 3 to 4 hours. The first step results in intermediate potassium benzoate (C6H5COOK) and alkaline medium potassium hydroxide formed from alkaline potassium permanganate. When potassium permanganate dissociates, it forms potassium ions K+ and permanganate ions MnO_4^- of purple colour. This K+ ion reacts with water in the mixture and forms the basic
In this research paper, I am going to investigate on how the concentration of Sodium Chloride (NaCl) solution affects the volume of the gases (Oxygen gas and Chlorine gas) at the anode during the electrolysis of sodium chloride solution. In my previous chemistry classes, I have learnt that concentration affects the majority of the gas produced at the anode. This made me wonder, how does the ratio of oxygen gas to chlorine gas produced at the anode vary at the electrodes. This made me formulate the research question: How does the concentration of Sodium Chloride solution affect the ratio of oxygen gas to chlorine gas produced at the anode during the electrolysis of Sodium Chloride solution? This area specifically interests me because it is still
The residual biomass was separated by filtration and washed with distilled water. For alginate extraction, the acidified algal biomass was suspended in 3% Na2CO3 solution at different alkali: alga ratio (20, 40, and 60 mL/g). The different extraction temperatures ranged from 25 to 45º C, and lasted for 1 to 3 h. For each experimental run, sodium alginate was collected by filtration and precipitated with absolute ethanol (1:2 v/v). The mixture was maintained at 4º C overnight. The precipitate was collected by vacuum filtration and allowed to dry at room temperature.
After cooling, the flask was weighed in an analytical balance. The experimental molar mass was 45.56g, which is closely identical to the 46.068g of the molar mass of ethanol. The percent error between the two values is 1.10%, the probable sources of error are the following: the size of the tiny hole in the aluminum foil, measurement of the temperature in the thermometer, and measurement of the initial volume of the flask. The recommendation for the experiment is to dry the flask completely before measuring the initial mass and to closely observe if the temperature of the water bath is
We can assume that the mass was very slightly decreased because of the gas created when the two were mixed but not significant enough for the scale to pick up. The mass staying the same shows that mass was not added or destroyed. The chemical equation is Mg+2 HCI→MgCl2+H2. Experiment 2 In experiment two a folded strip of white paper was placed on a circular piece of glass. The paper and glass had a combined mass of 29.1 grams before the experiment.