Fermentation is an ancient preservation method applied for fruits and vegetables. Most of the fermented foods and beverages are from household production for daily domestic consumption. In general, vegetables are dry salted or brined for fermentation process, which facilitate the production of flavor, control of undesirable microorganisms, extract nutrients and water and constitute soft tissue (Liu et al. 2011). The combination of salt with organic acids and biologically active compounds produced during fermentation period results in unique characteristics of the products and safety. Fresh fruits and vegetables naturally include a variety of microorganisms, mainly dominated by yeast and fungi. Although LAB are a small part of the microbiota …show more content…
2011). Although the main ingredients are cabbage and radish, the receipt may also include variety of vegetables such as carrot, mustard, green onion, parsley, ginger, red pepper garlic and fermented seafood (jeotgal) (Jung et al. 2011). Shredded cabbages are placed into a salt solution (5-7% for 12 h) and rinsed with water several times and drained. Then other ingredients is mixed with cabbage. Although ingredients and production methods are different, its fermentation is microbiologically similar to sauerkraut fermentation. Varieties of microorganisms have been identified during kimchi fermentation, mainly Leuconostoc spp. and Lactobacillus spp. (Table 5). In the first stage of the fermentation, Leuconostoc spp. constitute the dominant flora. In the later period, Leuconostoc spp. are inhibited by increasing lactic acid concentration and it is replaced by more acid-tolerant LAB, such as Lb. plantarum (Di Cagno et al. 2013). When the acidity reach to 0.5-0.6 %, optimum taste is obtained and the fermentation is stooped by refrigeration. Kimchi has a unique characteristic taste and usually served with cooked rice and soup (Rhee et al. 2011). As an alternative, it could be powdered to use in meat products as antimicrobial agent as well as flavoring (Kim et al. 2015). Lb. plantarum isolated from kimchi can show antibacterial and invasion protective activity, and modulate host immune response to promote a healthy gastrointestinal tract (Khan and Kang 2016). Moreover, several strains of bacteriocin
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided
Osmosis can be found in many forms throughout everyday human life. For example, meat and fruits are preserved in salt and sugar, respectively. In these cases, the solute concentration is higher than that of the bacterial cytosol. The result of this state is that the bacteria, which would normally consume and deteriorate the food, cannot survive as the water will leave their cells to enter the area of increased concentration. The osmosis studied in this experiment illustrates why the bacteria cannot survive, as the eggs in the hypertonic solution lost volume, as a bacteria in salt or sugar would (Janardan
The Unknown Identification Lab was an experiment that provided the opportunity to apply all the tests that were learned in the semester of lab, to identify the two bacterias that remain unknown. Gram- staining and two other tests will be used to identify the unknowns. This experiment is crucial to the understanding of each test, and can benefit in the ability to identify the characteristics of specific bacteria. Having a clearer understanding of the bacteria can further the research of bacteria for medicine, such as antibiotics. The understanding can also help the development of research in the environment.
In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
The bacteria, which is found in flies’ natural food source rotting fruit, was represented by the yeast, and the sugar was represented by the maple syrup. The purpose was to be able to identify which substances the flies were attracted to the most
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
This energy meets the needs for growth and maintenance of internal functions. Under anaerobic condition, yeast switches to fermentation which utilizes only about 5% of the energy contained in glucose and ethanol as the end product is produced. Acetic acid bacteria convert glucose to produce gluconic acid and ethanol to produce acetic acid giving it a sour flavor. The acetic acid production in turn induces the yeasts to produce ethanol and making alcohol available to bacteria. Both ethanol and acetic acid possess antimicrobial properties and thereby act against pathogenic bacteria and prevents contamination of the tea fungus (Liu et al., 1996).
Introduction - Research Question: How does the change in pH affect the fermentation of yeast and its effect on the product ethanol? Yeast: Yeast are unicellular microscopic organisms that are able to by budding and are used to convert sugars into alcohol and carbon dioxide. It is a member of the kingdom of fungi where currently there are over 1500 different species and strains of yeast.
I’ve spent several summers at the Brickner Microbiology Lab at Northwestern University. I first became involved with the lab the summer after my sophomore year of highschool. I worked as a paid lab technician. I preformed custodial duties and learned how to operate and maintain pieces of lab equipment. During this time I sat in on “Journal Club” paper review sessions and learned about the research projects running at the lab.
The difference or similarities between the gut microbiota data does not support a strong genetic inheritability, as
By Gram staining alone, it was safe to eliminate the three Gram positive bacteria that could have been assigned: S. epidermidis, M. luteus, and B. megaterium. The second step was to streak plate Unknown #10 to observe its macroscopic
, seeds included 4. Ginger- 1 tbsp. , freshly grated after peeling 5. Rice vinegar- 1 tbsp., unseasoned 6.
For example, fermentation occurs in yeast in order to gain energy by transforming sugar into alcohol. Fermentation is also used by bacteria, they convert carbohydrates into lactic acid. Ethanol fermentation is done by yeast and certain bacteria, when pyruvate is separated into ethanol and carbon dioxide. Ethanol fermentation has a net chemical equation: C6H12O6 (glucose) > 2C2H5OH (ethanol) + 2CO2 (carbon dioxide). This process of ethanol fermentation is used in the making of wine, bread, and beer.
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.