Inoculated the Lactococcus lactis ssp. lactis C2 culture onto the petri dish with bottom agar by using inoculation loop for streaking. The streaking was used to isolate the pure strain of Lactococcus lactis ssp. lactis C2 from the stock culture. The inoculation loop was sterilized in a fire for 5-7 seconds and then cooled to dip into the starting culture for microbes inoculation on the plate.
22.5 g of plate count agar powder was dissolved in a litre of sterile distilled water on the hot plate 2. pH of the solution was adjusted to 7.0 ± 0.2 by adding NaOH or HCl and was immediately transferred into the Schott bottle to be autoclaved at 121 ° C for 15 minutes 3. Prepared medium was stored in 4° C chiller Lauryl Sulphate Broth
Indole production test The ability of bacterial isolates to produce indole in broth was tested following the method described by Seeley and Vandermark, (1981). Each fresh isolate was inoculated to sterile trypton broth in test tubes and incubated at 37±1o C for 24-48 hours. After incubation, to these tubes 0.5 ml of kovac’s reagent was added and mixed well. Development of red ring in alcohol layer within few minutes was considered as positive test for indole production. Composition of Trypton broth medium Composition of Kovac’s reagent Trypton 10g Sodium chloride 5g Calcium chloride 1g Distilled water 1000ml PH 7.2 P- Dimethyl amino benzaldehyde
flask as the distillation flask. Use a 125 ml Erlenmeyer flask as the receiver. • Remove the thermometer and adapter from the assembly and place along-stem funnel in the still pot. Insert a small plug of absorbent cotton at the top of the funnel stem. • Carefully decant the solvent solution from the drying agent through the funnel into the flask.
The same method was used for other solvents dichloromethane (DCM), chloroform (CHCl3) and n-Butanol (nBuOH) and distilled water. These solvents and distilled water left were evaporated using the Freeze Dryer (GENEVAC LTO, EZ 2.3 ELITE). Each crude sample obtained from different solvents were weighed and tabulated in the table. The products were stored in refrigerator at -20°C until
The feed material was supplied by Lonmin Plc. This is the residue from the 3rd stage sulphuric acid leaching in the Lonmin BMR circuit. The wet material was kept in a container to avoid vaporisation and the loss of moisture to obtain the same weight for each leach test. Reagents All solutions where prepared with deionized water and grade A chemicals. The reagents used in this investigation were: Sodium hydroxide (NaOH) 50%-weight percent.
Briefly, the crude gum powder was boiled with 80% ethanol solution to deactivate enzymes and dissolve out low molecular weight carbohydrates with coloring matters. Thereafter, it was dispensed in deionized water and gently stirred overnight in a magnetic stirrer. The gum solution was allowed to stand for 12 h at room temperature to detach any undissolved matters. The gum solution was then filtered by triple folded muslin cloth and evaporated up to one third of the original volume using a rotary vacuum evaporator (Heidolph, Germany), cooled, precipitated with isopropanol and air dried. The end product was passed via sieve (no.
Palm oil industry is one of the strategic industry in tropical countries, such as Indonesia, Malaysia, and Thailand. Palm oil is used in a wide variety of food products such as cooking oil, shortenings, and margarine, whereas PKO is a raw material in the production of non-food products including soaps, detergents, toiletries, cosmetics, and candles. This industry is growing fast in accordance with the increasing demand of oil for food or energy. This large production scale influence the Indonesian economic growth. In the future, these industries will support national economic growth.
This particular molecule is related to university in addition to elastin in this system which keeps your bones, important joints, and veins accommodating. These pinto beans likewise contain vitamin B within them which supports inside generating strength for our physique of which endures the full day time along with develops mental performance cell/cognitive functionality. Lupini Pinto beans Health Benefits But not only tend to be lupini legumes high in proteins but also consist of fabric. They also have low fat, oil, as well as starchy foods articles causing them to be among the ideal beans. Ingesting lupini pinto beans will allow you to feel more voluminous much longer, supply you with additional electricity every day, in addition to burn calories.
viscida were collected from Kalakad villages in Tirunelveli District, Tamilnadu. The plant material was identified by St. Xavier’s college Herbarium (XCH) at Palayamkottai, Tirunelveli. The leaves and stem of P. viscida were a shade dried and powder with the aid of an electronic food processor. The 30 g of fine powdered materials was rolled in a filtered paper and kept in soxhlet apparatus with 250 ml of solvent (Petroleum ether, benzene, chloroform, methanol and Dis.H20 solvent) for the extract preparation at 24 h process. For antibacterial activity studies was carried by Whatman No.