Fish Food In Food

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UNIVERSITY OF AGRICULTURE, FAISALABAD DEPARTMENT OF ZOOLOGY, WILDLIFE AND FISHERIES (Synopsis for M.Phil.)
TITLE: Fish meal replacement by increasing the percentage of canola meal in diet for Labeo rohita supplemented with phytase and citric acid and their role in growth and nutrient digestibility.

Student Name: Muhammad Yar Khan
Registration No: 2014-ag-2377
Supervisor Name: Prof. Dr. Muhammad Afzal

Abstract This research work will be conducted to evaluate the impact of fish meal replacement with canola and supplemented with phytase and citric acid on growth and nutrient digestibility of Labeo rohita. This experiment conducted in lab with four experimental diets (Diet1, Diet2,
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By-products of plants are the sources of energy and protein for the formulation of environment friendly and economical aqua feeds (Cheng & Hardy, 2002).The demand is continuously rising because of high utilization of fish meal in feed industry. High demand of fish meal and the shortage of global production in feed industry have affected quality and price of fish meal. That’s why, in aquaculture industry it become an expensive nutritional source (Tacon, 1993).
Phytate enzyme present in nuts, oil seeds, legumes, and cereals, and has about 60 to 90% of total phosphate as phosphorus in case of plants (Raboy, 2003).Phytase enzyme improves the performance and fish growth fed on plant in L. rohita fingerlings and nutrient digestibility (Baruah et al., 2007a).
Phytase called an enzyme; chemically known as myoinositol hexaphosphate phosphohydrolase and belongs to Class 3(Hydrolases). Phytase may be present in plant ingredients or may be produced by microorganisms. The indigestible phytate enzyme is very specific to hydrolyze that is present in plant protein sources. Some fishes cannot produce this enzyme like mono gastric fishes and cannot hydrolyze the phytate. Moreover, supplementation of phytase in fish feeds has been generally reported to improve the utilization of plant phosphorus (P) by fish and the bioavailability (Cao et al.,
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(2007) performed an experiment to assess the effects of phytase supplementation on minerals digestibility to Labeo rohita fingerlings that were fed canola (30 percent) based diet. The experimental diet was consisted of 30 percent test ingredient and 70 percent reference diet. The chromic oxide was included 1 percent of concentration as non-digestible marker in the feed. The study results showed that the high digestibility (%) of Cu, Zn, P, Mg, Na, and K was observed at 750 FTU kg-1 level phytase supplementation and differed significantly (p<0.05) interaction between citric acid and phytase was observed to improve the minerals contents in the bones and body of fish. It was concluded that phytase and citric acid are supplements that are very effective to increase the minerals bioavailability from soybean meal based

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