Each sample of solution #3 being tested by the three reagents will have the most noticeable change in color in result of a positive reaction between solution #3 and the reagent. 5. We took three samples of three solutions, a positive control, and a negative control (fifteen test tubes total.) Each sample was tested by Benedict’s, Biuret’s, and Lugol’s Reagent for a reaction. The result of the reaction was then recorded in notes.
During this step, I observed that there were bubbles in the solution, especially at the bottom of the beaker. After adding the HLC, there solution had a slight yellow tint. Next, I mixed 0.529g of sodium acetate in 3mL of water and added 0.679g of acetic anhydride to the aniline solution and immediately added sodium acetate. The solution was cooled in an ice bath for fifteen minutes. During this time, I noticed the formation
The line of best fit gives the respiration rate of day-old seedlings as the concentration of NaCl they are exposed to increases. As NaCl Concentration increases the rate of cellular respiration decreases by .108 ppm CO2/g per second. This overall decrease throughout the data further supports our hypothesis. Discusion: The data collected in the experiment does support our hypothesis. By examining the data as a whole a trend of decreased cellular respiration in seedlings soaked in solutions with increased NaCl concentrations.
1.25mL of acetic acid and ferric chloride mixture was added to 2.5mL of the pure extract. Occurrence of blue-green color indicates the presence of glycosides. Test for Saponins. 10mL of distilled water was added to 5 mL of the pure extract the mixture was shaken in a graduated cylinder for 15minutes. Occurrence of layer of foam or bubbles indicates presence of saponins.
The sample was transferred to a 250 ml conical flask kept in water bath for alkali treatment. 75 ml of 17.5% caustic soda was measured using a measuring cylinder at 20°C. 15 ml of 17.5% NaOH was added and fibres were macerated gently with a flattened glass rod for 1 minute. 10 ml more NaOH was added and the solution was mixed for 45 seconds. 10 ml NaOH was again added and mixed for 15 seconds to make lump free slurry.
After 30 minutes, tubes were taken out and kept in ice-cold water for 30 minutes. These were centrifuged at 3000 rpm for 15 minutes. The absorbance of the supernatant was read at 540 nm at room temperature against appropriate blank. Blank consist of 1 ml distilled water, 0.5 ml of 30% TCA, and 0.5 ml of 0.8% TBA. TBARS values were expressed as n moles malonaldehyde (MDA)/mg protein.
3) Centrifuged at 2500 rpm for 12 mins. Upper hexane layer (supernatant) was transferred carefully into another test tube. 4) Evaporated the hexane under a stream of grade 1 nitrogen gas and added 100 µl of methanol to the residue left and vortexed for 1 min. 5) Injected 100 µl of extract in HPLC vials and closed properly. Standard curves and calculations- Retinol was quantified from standard curves peak area for each vitamin.
It was then washed off with ether after the drying process was finished and allowed 5-10 minutes for the drying of the ether solution. ?M HCl was added drop wise to tube 2 to neutralization, while testing the solution with litmus paper. A boiling stick was then added to the tube and heated cautiously to bring most of the solid carboxylic acid into solution. The tube was then allowed to cool slowly to room temperature then cooled in ice. The solvent was removed and the residue recrystallized from boiling water.
Dissolve the salt in 120 ml of tap water. Add 60 ml 6 M Hcl and stir the mixture with a glass rod. Add 24 g solid Nacl to the solution and stir the mixture for about 2 minutes. Support a 500 ml separatory funnel on a ring, close stopcock and then a clean beaker is placed beneath the exit tube. Transfer the aqueous solution from the beaker to the separatory funnel.