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Fusarium Oxycorum And Chickpea

1565 Words7 Pages

MicroRNAs in plant-pathogen interactions: cross-kingdom microRNA mediated gene regulation in Fusarium oxysporum and their host plant chickpea

1. Proposed area of research: Plant Science
2. Title of the proposed study: MicroRNAs in plant-pathogen interactions: cross-kingdom microRNA mediated gene regulation in Fusarium oxysporum and their host plant chickpea
3. Summary of proposed research work indicating
· Objectives (Preferably in bullet/numbered format)
1. Investigate the potential cross-kingdom miRNA-mediated gene regulation between Fusarium oxysporum and chickpea.
2. Identification and validation of target genes of chickpea miRNA in the Fusarium oxysporum genome
3. Identification and validation of target genes of Fusarium oxysporum miRNAs …show more content…

Insight into cross-kingdom regulation of miRNA between plant and pathogen
2. Molecular understanding of Fusarium oxysporum f. sp. ciceris-chickpea interaction
3. Identification of potential target genes to control Fusarium oxysporum infection
Importance of proposed research
Among the diverse pulses grown and consumed in India, chickpea (Cicer arietinum L.) is the major pulse crop accounted for around 45% of total pulse production. India alone produces 75 % of the global chickpea and additionally imports a large amount of chickpea to meet the increasing domestic requirements (http://www.aicrpchickpea.res.in/). The average yield of chickpea in India and globally is less than one 1t/ha which is far less than the estimated potential under optimum growing conditions. Among the several factors limiting yields, diseases cause significant yield losses in chickpea. The major diseases affecting chickpea are wilt and root rot complex caused by Fusarium oxysporum f. sp. ciceris, Fusarium solani, Rhizoctonia solani, Rhizoctonia bataticola, and Sclerotium rolfsii. Among them, Fusarium wilt is one of the devastating diseases affecting chickpea in India and several parts of the world. Therefore research on understanding the genetic and molecular basis of resistance to Fusarium wilt will allow development of novel biotechnological approaches to genetically improve chickpea resistance to fusarium wilt and thereby improve chickpea …show more content…

Genes involved in flowering time such as FRIGIDA-like protein 4a and Agamous-like MADS-box protein were also identified as potential targets. Genes involved in signal transduction (Cysteine-rich receptor-like protein kinase 10) and intramembrane cleaving protease (signal peptide peptidase) was also identified. Involvement of the target genes in stress response and observed correlation between flowering time and for Fusarium oxysporum resistance these genes are among the preferred candidate for the expression validation

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