Gardenia Lucid Analysis
Introduction
Gardenia is a genus of flowering plant of Rubiaceae family. Gardenia lucida Roxb., is one of the noted species of genus Gardenia. Gardenia lucida Roxb (Syn. Gardenia resinifera Roth) typically present in North-Eastern part of the India but also spread over other parts of world (Chaudhuri, 2007). The sticky resin discharge from the leaf buds of Gardenia lucida is in the form of yellow tears having an offensive smell and bitter taste (Suryanarayana et al., 2012). The resinous gum of Gardenia lucida is locally known as Dikamali gum.
Globally, Dikamali is used for ethanomedical purpose and folklore system of medicine for the treatment of a wide range of ailment viz dyspepsia, flatulence for cleaning foul ulcers and wounds, to keep off flies from wounds in veterinary practice. Current investigations have also revealed that Dikamali possesses a broad spectrum of biological activities such
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lucida (1.0 kg) was extracted with methanol (4 21L) by percolation at room temperature (25±5o). The methanol extract was dried in vacuo to give a residue (675.0g). The residue was suspended in water and subsequently partitioned between hexane, chloroform and n-butanol. Chloroform extract (180 g) was chromatographed over silica gel column eluting with a gradient system of hexane: ethylacetate. Fractions (236-306), eluted with hexane: ethylacetate (90:10) were afforded Gardenin-B (500 mg). Fractions (374-430) and Fractions (439-500) eluted with hexane: ethylacetate (85:15) yielded Gardenin-A (450mg). 5-Desmethylnobiletin (350mg) respectivily. Fractions (526-535) was subjected to reverse phase preparative HPLC (RP-C18, MeOH: H2O with 0.1% trifluoroacetic acid; 60:40, 3 mL/min) at 334 nm which yielded compound 4 Xanthomicrol (50 mg) (Retention time-12.5 minute). Fractions (577-582), eluted with hexane: ethylacetate (80:20) yielded Gardenin-D (150mg). Fractions (636-642), eluted with hexane: ethylacetate (80:20) afforded Gardenin-E
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