An endospore is a dormant of a bacterial cell. It is a non-reproductive structure that ensures survival of a bacterium through stressful environmental conditions. Unknown #76, using aseptic technique, was inoculated to a nutrient sporulation medium (NSM) plate. This concerns a selective medium that increases the initiation of endospore production. A spore-former would have green-pigmented endospore cells when looked at under the microscope. From the growth on the NSM, I smeared it aseptically to a wet slide. Slide was then left to be air-dried for about 10 minutes. It was important to heat fix the slide using a micro incinerator. The succeeding steps had to be taken with caution because the primary stain, malachite green, is toxic. Under the hood, the slide was covered with a properly cut size of paper towel. The slide was then stained and left to steam with malachite green. It was continuously followed up by applications of the stain so it may remain moist for 10 minutes. The slide was then rinsed and safranin was again used as a counterstain. Using oil immersion objective lens of the microscope, unknown #76 had only reddish-pink cells without any signs of spore formation. Thus the given unknown is a non-spore former. Bacillus subtilis was used for positive control and Escherichia coli for negative control for endospore
If I had been on the jury of this case against Pete Anderson I would have handed the jury the verdict that Pete Anderson was in fact guilty. I think that Pete Anderson illegally took archeological artifacts from public land. I think the fact that the location of the cronquistii coincides with the location of the looted Anasazi site, is an indication that Pete Anderson did in fact take the plants from this land area. The three types of daisy’s in the area were confertifolia, tortifolia, cronquistii. Regarding the morphology of these daisies, they each were able to be identified by the size of the “compositase” protein. However, after investigation through gel electrophoresis, the three kinds of plants were not identical. This relates to the
1. This experiment was performed using cells from 3 different species, Vicia faba (broad bean), Allium cepa (onion), and Coregonus clupeiformis (whitefish), which obviously have variability between them. Onions are bulb plants, meaning they have a ball of stored nutrients underneath the soil out of which the roots protrude, where the broad bean does not have a bulb, having most of its mass above the soil. The whitefish is of course an animal, entirely different from the plants, including in how the cell cycle is performed. A cleavage furrow forms instead of a cell plate to perform cytokinesis, and centrosomes are present in its mitotic cycle, unlike in plants. It should also be said, pertaining to this experiment, that whitefish travel to spawning grounds to reproduce, and that there is no parental care, which could have an effect on the rate of cell division in embryonic and larval whitefish. The data my group collected is somewhat similar to the average data, though it has some significant differences. The
Ans: The 8th lane shows up nothing which means hair fllicle DNA sample doesn't have DNA in it. The 9th lane showed up bright light colour which means Cauliflower DNA sample has DNA in it, but both 8th and 9th lanes are expected to show bright
Riss, T., Moravec, R., Niles, A., Duellman, S., Benink, H., Worzella, T., Minor, L. 2013. [Internet]. Cell Viability Assays. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences. [cited 2017 Feb 4]. Available from https://www.ncbi.nlm.nih.gov/books/NBK144065/
Sordaria fimicola is a microscopic fungal species that produces ordered tetrads. It is commonly used in classrooms because it lacks conidiospores, has a short generation time with matching genotype and phenotype, has known color genes that permit tetrad analysis, easily observable crossing over effects, and does not undergo spindle overlap. In S. fimicola, meiosis occurs in the ascus. The fungus is a haploid organism for the majority of its life. It only becomes diploid when mycelia of two unlike strains fuse. Mycelia are a network of long hyphae filaments which the fungus uses to form sex organs. The newly formed, diploid nucleus must go through mitosis to become haploid again. This will produce eight haploid ascospores held in the ascus.
In the experiment, the problem was the contaminants that were affecting the quality of the water samples. To fix this issue, three scientists had to determine the contaminants that were present in the samples. One sample was from the school sink and the second sample was from an unknown source. The scientists conducted many tests to figure out what pollutants were present in the water. In the experiment they used probes to test for pH, dissolved oxygen, total dissolved solids, conductivity, and turbidity. The probes hook up to the computer and collected the averages for each test. They collected this data and used it for later evaluation of the contaminates. They also tested for iron, copper, and chlorine with different tablets.
three phases G1, where the cells grows up to double it’s original size, S phase, where the dan is replicated , and G2 ,where the cells finishes it growing and prepares to go into prophase. Prophase is where the cell’s nuclear membrane dissolves. Chromatin tightly coils and condenses into chromosomes. Spindle fiber begins to appear and then the cell goes on to metaphase. During metaphase, the cell’s spindle fibers attach to the chromosomes and the chromosome aline in the middle of the cell. Anaphase occurs next in which the cell’s centromeres divide and the sister chromatids separate and move to the opposite sides of the cell. Then comes telophase in which the nuclear membrane begins to reform and the chromosome begin to decondense. Also the spindle fibers disappear. We end this
For this experiment, we studied how planarian worms would grow after being segmented. To begin this experiment, we filled up petri dishes half-way with spring water, this is so the planarian worms would not dehydrate and die during the two weeks we were observing them. Next, the planarian worm was picked up from its container with a pipette and then placed in a separate petri dish with some water. This took some time and I ended up having to use a pick to get the planarian worm off the inside walls of the pipette. Once the worm was in the petri dish, a razor was used to cut the worm in half, creating a “head” and “tail” segment of the worm. Since my worm was of a larger size, I did not feel the need to place it under a microscope and cut it
There are four main different categories of diseases, the first is infectious this can be passed on through person to person, this causes viruses, bacteria and fungus an example of this is thrush which is a fungus bacterium. Secondly there is the deficiency disease an example of this is anemia which is caused by lack of iron. There is also the hereditary disease an example of this disease is sickle cell and cystic fibrosis. Lastly there is the physiological disease an example of this is asthma and diabetes. 1.1