Gas Chromatography Principle

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Gas Chromatography
A sample to be analysed is injected into the chromatography instrument. The sample solution enters a gas stream which transports the sample into a separation tube which is also known as the column. Various components in the sample solution separated inside the column. GC has principles similar to fractional distillation. Both processes separate mixture based on boiling point. Fractional distillation does it on a larger scale whereas GC does it on a small scale
Discussion on analysis
This technique requires a stationary phase and a mobile phase. The mobile phase being the carrier gas is comprised of an inert gas such as helium, argon or nitrogen. The stationary
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Column chromatography is a method that purifies individual compounds from mixture of compounds. Applications on scale running for micrograms up to kilograms. The chromatography column is a glass tube with dimensions of 5mm-50mm in diameter and 5cm-1m in height. The column is fitted with a tap or filter at the bottom, this prevents the loss of the stationary phase. Similar to a burette. The principle of column chromatography is based on differential adsorption of substance by the…show more content…
This type of chromatography is used for the analysis and purification of low to moderate molecular weight, thermally liable molecules. It is the most effective method for the separation of chiral compounds. Principles are similar to high performance liquid chromatography however supercritical fluid chromatography uses carbon dioxide as the mobile phase. The entire chromatographic flow path must be pressurized, because supercritical phase represents a state in which liquid and gas properties converge. Supercritical fluid chromatography brings the advantages and strong aspects of HPLC and GC
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