Gel Purification
1. Why is gel purification important? What is it used for?
Gel-purification is a procedure that yields DNA freed from impurities such as salts, free nucleotides and enzymes, suitable for downstream applications
Gel purification is used to recover desired DNA fragments from agarose gels after electrophoretic separation. Also to turn the pure DNA into a plasmid vector
2. What buffer is the DNA suspended in, after pillow purification?
Tris Acetate (TAE) buffer
3. How is TOPO cloning different from traditional cloning? (Enzymes used?)
Traditional restriction enzyme cloning methods are time-consuming and tedious, and with their relatively low efficiencies, multiple colonies must be screened to find the insert of interest.
TOPO PCR cloning only
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