The first of the four test was a Gram-stain. Once the experiment was completed the slide was then placed under a microscope where it was then categorized as Gram-negative bacilli. Its pink color when viewed through the microscope indicated that the test was Gram-negative and its rod shape indicated the morphology was bacilli. With these results the next test to be conducted was the citrate utilization test. A citrate tube was inoculated with P. aeruginosa and incubated at 37 degrees Celsius at 24 hours.
The tube was placed back in incubation for 96 more hours to observe any more positives. 2.10 Catalase Test A trypticase soy agar plate was used and after incubation, four drops of 3% Hydrogen Peroxide was added to the plate to flow over the bacterial growth. A presence of bubbling was observed. 2.11 Starch Hydrolysis A starch agar plate was inoculated with a streak of the unknown bacteria and then incubated. On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids.
In this experiment, racemic 2-methylcyclohexanone was reduced using sodium borohydride as a nucleophile to give a diastereomeric mixture of cis and trans secondary alcohols. The products were analyzed for purity using IR spectroscopy and gas chromatography. 1.2 g of 2-methylcyclohexanone and 10 mL of methanol were combined in a flask and cooled in an ice bath. Two 100 mg portions of sodium borohydride were added to the flask and stirred. 5 mL of 3M sodium hydroxide, 5 mL of de-ionized water, and 15 mL of hexane were added to the reaction flask and stirred.
When testing one organism, for example Mycobacterium tuberculosis, the dilution is prepared in agar slopes but at this time it is necessary to prepare another identical set to be inoculated with the organism which is control. To make dilution a small volume of water is used and then the dilute is added to agar that melted and then cooled to 60degrees C. If chocolate agar is required blood is added, and the medium must be heated before addition of the antibiotic. Petri dishes with 90mm diameter are convenient to be used and one ml of the desired drug dilutions is added to 19ml of the broth. Agar dilution factor must be allowed in the first calculation as follows: • The final volume of the medium in the plate equals 20ml • Top concentration of the antibiotic equals 64mgL • Drug total amount equals 1280 microgram is added to 1ml water • 2mls of 1280microgram per ml is required to start the dilution equals 2560micrograms in 2mls. • 1.28mls of 2000micrograms per ml ± 0.72ml of water.
In this lab, genes for a fluorescent green protein (GFP) and antibacterial resistance (ARG) were inserted into E. coli bacteria. E. coli bacteria was resuspended in an ice-cold CaCl2 solution. DNA containing GFP and ARG was added to half of the cells before they were “heat shocked” in an ice bath and hot water. The heat shocking made the bacteria’s cell membrane more porous, so the DNA could enter. Recovery broth was added to the cell suspension, and the bacteria was placed in warm water for about thirty minutes (see Results and Discussion, paragraph 2).
We then added 10cm3 ethanoic anhydride to the salicylic acid and swirled the contents, this mixes together the two chemicals. We then added 5 drops of concentrated sulphuric acid to the flask and thoroughly swirled the mixture, this creates the solution that makes the aspirin. We then warmed the flask for 20 minutes in a 400cm3 beaker of hot water which was approximately 60°C, we made sure the flask did not go above 65°C because this could have caused the contents to evaporate. Part 2: Using a 25cm3 measuring cylinder we measured out 15cm3 of ethanol into a boiling tube and then prepared a beaker half filled with hot water at approx. 75°C, we got this temperature by filling the beaker with cold water and slowly adding boiling water from a kettle until we reached the right temperature.
Wash with 95% ethanol and wash with water after 15 seconds. 7. Stained counterstain with fuchsine and wash with water after 60 seconds. 8. Remove excess water from the surface of the gram stained glass slide and observed under 1000X oil immersion microscope.
We also used ice to see whether unknown compound will dissolve in the ice. We used 3ml of ice and 0.5 g of unknown and 1-gram sodium carbonate and 1g unknown carbonate in 10 ml H20. when we first mixed ice with unknown it melts then we used sodium carbonate and unknown carbonate it forms white precipitate. for the final test we used 1-gram sodium bicarbonate and unknown with 15 ml of H20 then it bubbles up. After we done with all other test, we like to see the PH of the sodium bicarbonate and unknown, its initial temperature is 20 and the final temperature was 24 and then PH paper turn blue and its has the PH of 8-9 and its very
Objective The objective of the experiment was to measure and analyze the reaction rate of tert-butyl chloride with sodium hydroxide, and plot it in a graph in order to observe the rate. Procedure Part A- Measurement of the SN1 Reaction Rate of Tert-Butyl Chloride • 100 mL of a solution of propan-2-ol and water (1:1 ratio) were collected from a container provided by the instructor, and they were placed in a 250 mL Erlenmeyer flask, which was subsequently cork-stoppered. Afterwards, 150 mL of NaOH were acquired and placed in a beaker, and in turn this 150 mL sample of NaOH was used to fill a 50 mL buret to the highest mark, and this buret was attached to a butterfly clamp attached to a ring stand in preparation for a future step. In a 100 mL
I let the alcohol flow on 45-degree angle slide within 15 seconds and wash it with water to remove colors on the surface. Lastly, the unknown is once again dyed with safranin for 1 minute then wash it off with water for the last time and dry it using bibulous paper. After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster.