MATERIALS
Gellan Gum was procured from Sigma (USA), Calcium Chloride dihydrate and CuSO4, 5H2O are supplied by Merck, India. All other reagents were of analytical grade.
INSTRUMENTS
Photo calorimeter (EI Digital photo colorimeter), mechanical shaker (Uptin 2MLH Magnetic Stirrer), pH meter (Toshiba, India)
EXPERIMENTAL
Preparation of new biomaterial hydrogel
1. 10 ml distilled water is measured by micropipette and poured in a small beaker.
2. Gellan gum (100mg) is effectively weighed.
3. The measured amount of the gellan gum is then added very slowly to the beaker while stirring at constant rate with magnetic stirrer.
4. The stirring process is continued till the sample powder gets properly dissolved within the volume provided. This takes about 30-45 minutes in average.
5.
…show more content…
Constant stirring while mixing causes development of gas bubbles in the viscous preparation. So kept for 15 minute to assure the layering of gas bubbles out.
6. After that 20ml of 0.5M calcium chloride dehydrate solution is taken with accurate measurement and placed with a magnetic stirrer and rotation is maintained within 100-200rpm to prevent hindrance in the crosslinking process.
7. Now viscous solution is pulled into a syringe of 10ml and drop wise made to fall into the crosslinking solution. The needle of the syringe is hold at distance 1.5-2cm above the calcium chloride solution.
8. When all the polymer solution is cross linked to small micro beads, it is washed well with distill water to remove the adherence of extra calcium chloride on the surface of prepared beads.
9. Then wet mass of beads is measured in an analytical balance using butter paper. Weight of the beads obtained is deducted with the mass of the paper as it also contains some absorbed water molecule.
10. Measured beads were placed in petry dishes. It is allowed to dry well for a day and again the weight of dry beads is measured. Then the dry beads were kept in desiccators until further
After I rinsed the copper I transferred it to a watch glass that had been baked for 20 minutes so it would not contain any liquid so as to not counteract the experiment. After I put the copper on this watch glass I baked the copper in the oven for 20 minutes so there would be no liquid left in that to mess up the mass. After 20 minutes I removed the watch glass with the copper in it and weighed itm making sure to weigh the watch glass
In the lab “All That Glitters” the objective that was focused on during the lab was calculating the density, volume and mass of various substances. The method that was used in finding the volume of the samples is called the displacement method. This is a process where the volume of the water in the graduated cylinder is calculated before and after the sample is placed. In this lab, the goal of the experiment was to identify and come to consensus about what the unknown substance might be. For this experiment, the required materials were ten pre and post pennies, unknown sample, graduated cylinder, weigh boat, water, paper towels and a weighing scale.
Then the flask was filled the rest of the way with distilled water to the mark. Similar steps were taken for the rock solution. The rock solution from the prior lab was filtered into a volumetric flask (100mL), then 15 M NH4¬OH (8mL) was added to the flask. After that, the flask was filled to the mark with distilled water. Both flasks were then swirled to combine the solution
To calculate the experimental mass the substance of each bag and the bag its self was measured using a balance. After gathering the mass subtract the mass of the empty bag to the mass of the unknown substance, in order to just have the mass of the substance. Afterward the mass of the unknown substance was divided by the number of moles recorded on the bag of the substance. The measurements are displayed on the table
Advanced Biology Photosynthesis Lab Report By Cheryl G11 Introduction & Background Photosynthesis is the process by which light energy is converted to chemical energy. It occurs within each chloroplast in most of the green plants, and Carbon dioxide is one of the main components of this process. Plants use photosynthesis to produce their own food (glucose), some water and the oxygen that we need.
The dehydration of 2-methylcyclohexanol takes place at the bottom of the Hickman still. As the Hickman still heats up within the sand bath, the products evaporate and travel higher up in the still where they condense into a liquid and fall within the collection ring, thus separating the product from the remaining water. Drierite (CaSO4) is also added as a drying agent to absorb any leftover water within the product. The purity of the product will then be analyzed with infrared spectroscopy, paying attention to OH peak if it is present. Chemical Reactions: Data and Observations: Material Volume Mol.
Start timing the reaction. For the next 5 minutes, record the observed data at each minute (0, 1, 2, 3, 4, 5) Use the chart to observe the color. pH Procedure: Making sure the materials are clean from previous experiment, gather all materials.
I. Purpose: To experimentally determine the mass and the mole content of a measured sample. II. Materials: The materials used in this experiment a 50-mL beaker, 12 samples, a balance and paper towels. III.
The overall purpose of this lab was to develop a lab procedure in order to separate and measure the mass of each containment obtained the provided sample. In addition, this experiment was conducted in order to provide the EPA with a plan to remove all contaminants from a heterogenous mixture which purifies the water, making it accessible for the society. Furthermore, the sample consisted of the following contaminants, sand, rock, wood, plastic, salt, water, and an unknown metal. When it came to separating the contaminants, the wood and plastic were taken out through the use of tweezers, while the rocks were separated by decanting the mixture of sand and rocks from the water.
Experiment 2 Report Scaffold (Substitution Reactions, Purification, and Identification) Purpose/Introduction 1. A Sn2 reaction was conducted; this involved benzyl bromide, sodium hydroxide, an unknown compound and ethanol through reflux technique, mel-temp recordings, recrystallization, and analysis of TLC plates. 2. There was one unknown compound in the reaction that was later discovered after a series of techniques described above.
⋅ 5H2O, which has about 36.0%, and CuCl2 ⋅5H20 (21.17%). Materials: Ring stand, ring clamp, evaporating dish, Bunsen burner, clay triangle, crucible tongs, electronic balance, sample of hydrated salt. Methods:
IV. Data and observations Mass of beaker (g) 174.01 Mass of beaker + NaOH pellets (g) 174.54 Mass of NaOH pellets 0.53 TRIAL 1 TRIAL 2 Mass of potassium acid phtalate (KHP) (g) 0.15 0.15 final buret reading (ml) 30.75
A small amount of sand was added after the layer of cotton. After that, a layer of silica filled almost 1/3 of the column. Finally, another small amount of sand was added just above the silica. The column was given a little tap with an aspirator to make the silica more compact. Figure 2.